韩国过敏患者Der f23和Der p23的ige致敏性和交叉反应性。

IF 2.3 4区 医学 Q3 ALLERGY
Myung-Hee Yi, Tai-Soon Yong, Chung-Ryul Kim, Kyoung Yong Jeong, Ju Yeong Kim
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引用次数: 0

摘要

背景:室内尘螨(HDM)是过敏原的主要来源,迄今已鉴定出30多种HDM过敏原。最近,Der p23被报道为与过敏反应的严重程度有关的主要过敏原。目的:研究韩国过敏患者Der f23和Der p23的ige致敏性和交叉反应性。方法:利用酵母毕赤酵母表达系统制备重组Der f23和Der p23。采用酶联免疫吸附试验(ELISA)检测194例韩国hdm致敏患者血清中Der f23和Der p23的IgE结合活性。采用竞争性ELISA法测定Der f23与Der p23的交叉反应性。结果:194例hdm过敏患者中,对rderf23和rderp23的IgE反应率分别为45.36%(88/194)和43.81%(85/194)。10例患者血清的竞争性ELISA结果显示,rDer p23抑制了86.1%的rDer f23 IgE反应性,rDer f23抑制了61.1%的rDer p23 IgE反应性。结论:第23组HDM变应原derf23和derp23表现为中度致敏,韩国患者对其过敏的比例分别为45.36%和43.81%。两种过敏原之间存在显著的IgE交叉反应性。这些发现可以促进成分分辨诊断和过敏原特异性免疫治疗在未来的发展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
IgE-sensitization and cross-reactivity of Der f 23 and Der p 23 in Korean patients with allergy.

Background: House dust mites (HDM) are a major source of allergens, and more than 30 HDM allergens have been identified to date. Recently, Der p 23 was reported to be a major allergen that is related to the severity of allergic responses.

Objective: This study aimed to characterize IgE-sensitization and cross-reactivity between Der f 23 and Der p 23 in Korean patients with allergy.

Methods: We produced recombinant Der f 23 and Der p 23 using a yeast Pichia expression system. The IgE binding activity of Der f 23 and Der p 23 was evaluated by enzyme-linked immunosorbent assay (ELISA) using sera from 194 Korean HDM-sensitized patients. The cross-reactivity between Der f 23 and Der p 23 was then assayed using competitive ELISA.

Results: Among the 194 HDM-allergic patients, IgE reactivity to rDer f 23 and rDer p 23 was observed in 45.36% (88/194) and 43.81% (85/194) of the samples, respectively. Competitive ELISA with pooled serum from 10 patients revealed that rDer p 23 inhibited 86.1% of the rDer f 23 IgE reactivity and rDer f 23 inhibited 61.1% of the rDer p 23 IgE reactivity.

Conclusions: Group 23 HDM allergens, Der f 23 and Der p 23, show moderate sensitization, with 45.36% and 43.81% of Korean patients with allergy reacting to them, respectively. Significant IgE cross-reactivity was observed between the two allergens. These findings can facilitate the development of component-resolved diagnosis and allergen-specific immunotherapy in the future.

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来源期刊
CiteScore
12.80
自引率
0.00%
发文量
74
审稿时长
>12 weeks
期刊介绍: The Asian Pacific Journal of Allergy and Immunology (APJAI) is an online open access journal with the recent impact factor (2018) 1.747 APJAI published 4 times per annum (March, June, September, December). Four issues constitute one volume. APJAI publishes original research articles of basic science, clinical science and reviews on various aspects of allergy and immunology. This journal is an official journal of and published by the Allergy, Asthma and Immunology Association, Thailand. The scopes include mechanism, pathogenesis, host-pathogen interaction, host-environment interaction, allergic diseases, immune-mediated diseases, epidemiology, diagnosis, treatment and prevention, immunotherapy, and vaccine. All papers are published in English and are refereed to international standards.
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