食物限制诱导兔卵巢卵泡发生、细胞增殖、凋亡和调节肽产生的变化。

IF 2.7 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Animals Pub Date : 2025-04-30 DOI:10.3390/ani15091282
Imane Hadjadj, Zuzana Fabova, María-Luz García, Iván Agea, Barbora Loncová, Martin Morovic, Peter Makovicky, María-José Argente, Alexander V Sirotkin
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引用次数: 0

摘要

本研究旨在探讨食物限制对家兔卵巢功能的影响。8只雌性进行自由采食(NF), 8只进行50%限食(RF)。一个月后,所有雌性都被安乐死。测量卵巢和子宫角的重量和长度。对卵巢的代表性部位进行卵泡发生的组织形态学分析。分离颗粒细胞,评估细胞活力、增殖(PCNA、cyclin B1和brdu阳性细胞的积累)、凋亡(bax、caspase 3和DNA片段化的积累)。采用纳米HPLC-Chip-MS/MS方法对颗粒细胞进行蛋白质组学分析。采用ELISA法检测卵巢细胞和颗粒细胞对雌二醇和孕酮的释放。RF组卵巢和子宫角重量低于NF组。RF组卵泡和卵母细胞直径、卵膜和颗粒细胞厚度均高于NF组。RF显示含有bax和caspase 3的细胞百分比较低,DNA片段化细胞的发生率较低,雌二醇和黄体酮含量较低。RF具有较高的BrdU掺入率,含有PCNA和细胞周期蛋白B1的细胞比例较高,而活细胞百分比较低。RF比NF产生更多的特异性蛋白质,包括参与细胞分化、增殖/分裂、有丝分裂细胞周期和gtp酶活性的肽。综上所述,限食可以通过以下途径激活生殖:(1)对生长中的原始卵泡进行选择,(2)对排卵前卵泡进行更好的转化,(3)促进卵母细胞生长,(4)增加颗粒细胞增殖,减少细胞凋亡,(5)改变卵巢分泌活性,(6)改变多肽数量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Food Restriction Induces Changes in Ovarian Folliculogenesis, Cell Proliferation, Apoptosis, and Production of Regulatory Peptides in Rabbits.

The aim of this study is to examine the influence of food restriction on rabbit ovarian functions. A total of eight females were fed ad libitum (NF), while eight females were subjected to 50% food restriction (RF). One month later, all females were euthanized. Weights and lengths of ovaries and uterine horns were measured. Representative parts of the ovaries were subjected to histomorphometry analysis of folliculogenesis. Granulosa cells were isolated and cell viability, proliferation (accumulation of PCNA, cyclin B1, and BrdU-positive cells), apoptosis (accumulation of bax, caspase 3, and DNA fragmentation) were evaluated. Granulosa cells were subjected to proteomic analysis by using the nano HPLC-Chip-MS/MS method. Estradiol and progesterone release by ovarian and granulosa cells was assessed by ELISA. Ovarian and uterine horn weights were lower in RF than NF. The diameter of follicles and oocytes and the thickness of the theca and granulosa cells were higher in RF than NF. RF showed a lower percentage of cells containing bax and caspase 3, occurrence of DNA fragmented cells, and estradiol and progesterone. RF had higher incorporation of BrdU, a higher proportion of cells containing PCNA and cyclin B1, and a lower percentage of viable cells. RF produced more specific proteins than NF, including peptides involved in cell differentiation, proliferation/division, mitotic cell cycle, and GTP-ase activity. In conclusion, food restriction can activate reproduction by (1) selection of the growing primordial follicles, (2) better transformation of secondary to preovulatory follicles, (3) increasing growth of oocytes, (4) increasing proliferation and decreasing apoptosis in granulosa cells, (5) changes in ovarian secretory activity, and (6) changes in the number of peptides.

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来源期刊
Animals
Animals Agricultural and Biological Sciences-Animal Science and Zoology
CiteScore
4.90
自引率
16.70%
发文量
3015
审稿时长
20.52 days
期刊介绍: Animals (ISSN 2076-2615) is an international and interdisciplinary scholarly open access journal. It publishes original research articles, reviews, communications, and short notes that are relevant to any field of study that involves animals, including zoology, ethnozoology, animal science, animal ethics and animal welfare. However, preference will be given to those articles that provide an understanding of animals within a larger context (i.e., the animals'' interactions with the outside world, including humans). There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental details and/or method of study, must be provided for research articles. Articles submitted that involve subjecting animals to unnecessary pain or suffering will not be accepted, and all articles must be submitted with the necessary ethical approval (please refer to the Ethical Guidelines for more information).
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