[大鼠创伤性脑损伤后半暗区光学相干断层血管造影及微血管密度定量研究]。

Q3 Medicine
北京大学学报(医学版) Pub Date : 2025-04-18
Peng Zhong, Xiaodan Hu, Zhenzhou Wang
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引用次数: 0

摘要

目的:利用光学相干断层扫描血管成像(OCTA)观察创伤性脑损伤(TBI)大鼠半暗区微血管损伤及修复的动态变化。方法:对体重下降所致脑外伤后开颅大鼠置入透明封闭颅窗。TBI组和对照组大鼠术后第1天均行头部MRI检查,d0、d2、d4、d6、d8通过颅窗OCTA测量脑皮质微血管密度变化。术后第2天,选取两组相同数量的大鼠,用吡莫硝唑(缺氧指示物)完成脑组织免疫组化染色。结果:MRI T2W1和免疫组化染色显示,创伤脑组织水肿和缺氧深度延伸至整个皮层。OCTA显示两组大鼠术后皮质表面静脉明显扩张、弯曲,d8恢复至术后日水平。两组大鼠有效灌注微血管密度均在短暂下降后逐渐恢复,TBI组由d0时的39.38%±4.48%降至d2时的27.84%±6.01%,显著低于d0、d6、d8时(P < 0.05)。d8时最高,为61.71%±7.69%,显著高于d0、d2和d4 (P < 0.05)。对照组d0时为44.59%±7.78%,d2时为36.69%±5.49%,显著低于d0、d6、d8时(P < 0.05)。d8最高,为51.92%±5.96%,显著高于d2和d4 (P < 0.05)。两组比较,TBI组d2时有效灌注微血管密度显著低于对照组(P=0.021), d8时显著高于对照组(P=0.030)。结论:OCTA可作为脑外伤大鼠大脑皮层有效灌注微血管的成像和测量方法。创伤后创面半暗区有效灌注微血管密度由下降逐渐恢复,并在第8天显著升高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Optical coherence tomography angiography and microvessel density quantification in penumbra after traumatic brain injury in rats].

Objective: To observe the dynamic changes of microvascular injury and repair in the penumbra of traumatic brain injury (TBI) rats with effective cerebral perfusion microvascular imaging using optical coherence tomography angiography (OCTA).

Methods: Transparent closed cranial windows were placed in craniotomy rats after TBI caused by weight drop. All the rats in TBI group and control group underwent head MRI examination on the first postoperative day, and the changes of cerebral cortical microvessel density were measured by OCTA through cranial windows on d0, d2, d4, d6, and d8. On the second day after the operation, the same number of rats in the two groups were selected to complete the immunohistochemical staining of brain tissue with pimonidazole, an indicator of hypoxia.

Results: MRI T2W1 and immunohistochemical staining demonstrated that edema and hypoxia in the traumatic brain tissue extended deeply throughout the entire cortex. OCTA showed that the cortical surface veins of the rats in both groups were significantly dilated and tortuous after operation, and recovered to the postoperative day level on d8. The effective perfusion microvessel density of the rats in both groups gradually recovered after a temporary decrease, and the TBI group decreased from 39.38%±4.48% on d0 to 27.84%±6.01% on d2, which was significantly lower than that on d0, d6, and d8 (P < 0.05). The highest value was 61.71%±7.69% on d8, which was significantly higher than that on d0, d2, and d4 (P < 0.05). The control group decreased from 44.59%±7.78% on d0 to 36.69%±5.49% on d2, which was significantly lower than that on d0, d6, and d8 (P < 0.05). The highest value was 51.92%±5.96% on d8, which was significantly higher than that on d2, and d4 (P < 0.05). Comparing the two groups, the effective perfusion microvessel density in the TBI group was significantly lower than that in the control group on d2 (P=0.021), and significantly higher than that in the control group on d8 (P=0.030).

Conclusion: OCTA can be used as a method of imaging and measurement of effective perfusion microvessels in the injured cerebral cortex of TBI rats. After TBI, the effective perfusion microvessel density in the wound penumbra gradually recovered after decreasing, and increased significantly on d8.

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来源期刊
北京大学学报(医学版)
北京大学学报(医学版) Medicine-Medicine (all)
CiteScore
0.80
自引率
0.00%
发文量
9815
期刊介绍: Beijing Da Xue Xue Bao Yi Xue Ban / Journal of Peking University (Health Sciences), established in 1959, is a national academic journal sponsored by Peking University, and its former name is Journal of Beijing Medical University. The coverage of the Journal includes basic medical sciences, clinical medicine, oral medicine, surgery, public health and epidemiology, pharmacology and pharmacy. Over the last few years, the Journal has published articles and reports covering major topics in the different special issues (e.g. research on disease genome, theory of drug withdrawal, mechanism and prevention of cardiovascular and cerebrovascular diseases, stomatology, orthopaedic, public health, urology and reproductive medicine). All the topics involve latest advances in medical sciences, hot topics in specific specialties, and prevention and treatment of major diseases. The Journal has been indexed and abstracted by PubMed Central (PMC), MEDLINE/PubMed, EBSCO, Embase, Scopus, Chemical Abstracts (CA), Western Pacific Region Index Medicus (WPR), JSTChina, and almost all the Chinese sciences and technical index systems, including Chinese Science and Technology Paper Citation Database (CSTPCD), Chinese Science Citation Database (CSCD), China BioMedical Bibliographic Database (CBM), CMCI, Chinese Biological Abstracts, China National Academic Magazine Data-Base (CNKI), Wanfang Data (ChinaInfo), etc.
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