中和剂辅助富集和pmax - qpcr优化大蒜颗粒中沙门氏菌检测

IF 1.9 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY
Bingkui Li, Yuxin Zheng, Yawen Huang, Jiahao Ji, Shaoting Li, Hongmei Zhang
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引用次数: 0

摘要

低水活度(aw)的香料通常含有抗菌物质,这使得使用传统培养方法检测沙门氏菌变得复杂。这些物质可以使沙门氏菌进入可存活但不可培养(VBNC)状态,使其无法用基于培养的方法检测到。本研究旨在通过减轻抗菌物质的抑制作用来增强大蒜颗粒中沙门氏菌的富集肉汤。本研究通过添加3倍缓冲蛋白胨水来提高培养基的缓冲能力,添加硫酸镁和丙酮酸钠来促进细菌的自我修复和恢复,添加dl -二硫苏糖醇(DTT)和玉米油来中和大蒜中的抗菌物质,对富集肉汤进行了优化。用沙门氏菌对大蒜颗粒进行了人工污染,并进行了为期2周的干燥处理,以模拟食品储存期间的低温度条件。结果表明,在改性缓冲蛋白胨水(mBPW)肉汤中添加DTT和玉米油作为中和剂,可显著提高大蒜颗粒中沙门氏菌的检测能力,在初始污染水平低至5 CFU/g时即可检出沙门氏菌。相比之下,沙门氏菌在富集过程中没有添加中和剂是检测不到的。此外,对优化后的mBPW肉汤进行了除大蒜颗粒外的香料检测,结果表明该肉汤在不添加中和剂的情况下对八角茴香和黑胡椒中的沙门氏菌有检测效果。值得注意的是,本研究表明,中和剂辅助富集和pmax - qpcr的结合成功地检测了大蒜颗粒中的活菌沙门氏菌。整个过程可在约26小时内完成,证明了该方法检测活细胞的快速和有效。该方法为大蒜颗粒中沙门氏菌的检测提供了一种快速、灵敏的方法,在类似的抑菌食品基质中具有广阔的应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Optimization of Salmonella Detection in Garlic Granules Using Neutralizer-Aided Enrichment and PMAxx-qPCR.

Spices with low water activity (aw) often contain antibacterial substances that complicate the detection of Salmonella using traditional culture methods. These substances can drive Salmonella into a viable but nonculturable (VBNC) state, rendering it undetectable with culture-based methods. This study aimed to enhance the broth for enriching Salmonella in garlic granules by mitigating the inhibitory effects of antibacterial substances. This study optimized the enrichment broth by using 3× buffered peptone water to increase the buffering capacity of the medium, adding magnesium sulfate and sodium pyruvate to promote the self-repair and recovery of bacteria, and adding DL-dithiothreitol (DTT) and corn oil to neutralize the antimicrobial substances in garlic. Garlic granules were artificially contaminated with Salmonella and subjected to a 2-week desiccation process to simulate low aw conditions during food storage. The results showed that adding DTT and corn oil as neutralizers in the modified buffered peptone water (mBPW) broth significantly enhanced the detection capability of Salmonella in garlic granules, enabling detection of Salmonella at initial contamination levels as low as 5 CFU/g. In contrast, Salmonella was undetectable without the addition of neutralizers during enrichment. In addition, the optimized mBPW broth was tested on spices other than garlic granules, demonstrating its effectiveness (without neutralizers) in detecting Salmonella in star anise and black pepper. Notably, this study demonstrated that the combination of neutralizer-aided enrichment and PMAxx-qPCR successfully enabled the detection of viable Salmonella in garlic granules. The entire process can be completed in approximately 26 h, demonstrating the method's swiftness and effectiveness in detecting viable cells. This approach provides a fast and sensitive method for the detection of Salmonella in garlic granules, with promising potential for application in similar inhibitory food matrices.

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来源期刊
Foodborne pathogens and disease
Foodborne pathogens and disease 医学-食品科技
CiteScore
5.30
自引率
3.60%
发文量
80
审稿时长
1 months
期刊介绍: Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.
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