{"title":"发酵灯盏花黄酮类化合物:抗伪狂犬病毒的体内外作用及机制。","authors":"Ying Zhang, Ting Li, Chunkun Yang, Qiong Pan, Changxu Pan, Xue Zhang, Ying Zhang, Xianghua Shu, Zheng Wang, Zhenghong He, Zichen Qu, Chunlian Song","doi":"10.3389/fvets.2025.1562879","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong><i>Erigeron breviscapus</i> exhibits anti-inflammatory properties, protects neuronal cells and enhances immune function. Modern traditional Chinese medicine fermentation techniques can increase the bioactive compound content in <i>Erigeron breviscapus</i>. However, its potential therapeutic effects against the porcine pseudorabies virus (PRV) remain unclear.</p><p><strong>Methods: </strong>A PRV infection model was established in mouse trigeminal ganglion (TG) cells to determine the optimal antiviral mode of action of flavonoids from fermented <i>Erigeron breviscapus</i> (FEBF). Additionally, a PRV-infected rat model was developed to evaluate the <i>in vivo</i> antiviral efficacy of FEBF.</p><p><strong>Results: </strong>FEBF demonstrated a higher protective rate and a lower viral copy number compared to unfermented <i>E. breviscapus</i> flavonoids (EBF). The protective effect was most pronounced under toxicological and inhibitory conditions, surpassing the blocking effect. PRV infection upregulated TLR4, MyD88, and NF-κB p65 protein expression during the pre-infection phase, followed by their downregulation after 12 h. FEBF regulated PRV-induced changes in protein expression, restoring them to near-normal levels by 36 h. <i>In vivo</i> assessments of pathological injury, PRV viral load, neuronal count, and neuronal apoptosis indicated that FEBF provided superior neuroprotection compared to both Minocycline (MINO), a broad-spectrum neuroprotective drug, and unfermented <i>EBF.</i> Mechanistic studies further revealed that FEBF modulated microglial polarization and regulated the inflammatory cytokines IL-6, TNF-<i>α</i>, IL-4, and IL-10.</p><p><strong>Conclusion: </strong>These findings demonstrate that FEBF exhibits significant antiviral effects against PRV in both <i>in vitro</i> and <i>in vivo</i> models. FEBF represents a promising candidate for the development of anti-PRV therapeutics.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1562879"},"PeriodicalIF":2.6000,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12042226/pdf/","citationCount":"0","resultStr":"{\"title\":\"Fermented <i>Erigeron breviscapus</i> flavonoids: anti-pseudorabies virus efficacy and mechanisms <i>in vitro</i> and <i>in vivo</i>.\",\"authors\":\"Ying Zhang, Ting Li, Chunkun Yang, Qiong Pan, Changxu Pan, Xue Zhang, Ying Zhang, Xianghua Shu, Zheng Wang, Zhenghong He, Zichen Qu, Chunlian Song\",\"doi\":\"10.3389/fvets.2025.1562879\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong><i>Erigeron breviscapus</i> exhibits anti-inflammatory properties, protects neuronal cells and enhances immune function. Modern traditional Chinese medicine fermentation techniques can increase the bioactive compound content in <i>Erigeron breviscapus</i>. However, its potential therapeutic effects against the porcine pseudorabies virus (PRV) remain unclear.</p><p><strong>Methods: </strong>A PRV infection model was established in mouse trigeminal ganglion (TG) cells to determine the optimal antiviral mode of action of flavonoids from fermented <i>Erigeron breviscapus</i> (FEBF). Additionally, a PRV-infected rat model was developed to evaluate the <i>in vivo</i> antiviral efficacy of FEBF.</p><p><strong>Results: </strong>FEBF demonstrated a higher protective rate and a lower viral copy number compared to unfermented <i>E. breviscapus</i> flavonoids (EBF). The protective effect was most pronounced under toxicological and inhibitory conditions, surpassing the blocking effect. PRV infection upregulated TLR4, MyD88, and NF-κB p65 protein expression during the pre-infection phase, followed by their downregulation after 12 h. FEBF regulated PRV-induced changes in protein expression, restoring them to near-normal levels by 36 h. <i>In vivo</i> assessments of pathological injury, PRV viral load, neuronal count, and neuronal apoptosis indicated that FEBF provided superior neuroprotection compared to both Minocycline (MINO), a broad-spectrum neuroprotective drug, and unfermented <i>EBF.</i> Mechanistic studies further revealed that FEBF modulated microglial polarization and regulated the inflammatory cytokines IL-6, TNF-<i>α</i>, IL-4, and IL-10.</p><p><strong>Conclusion: </strong>These findings demonstrate that FEBF exhibits significant antiviral effects against PRV in both <i>in vitro</i> and <i>in vivo</i> models. FEBF represents a promising candidate for the development of anti-PRV therapeutics.</p>\",\"PeriodicalId\":12772,\"journal\":{\"name\":\"Frontiers in Veterinary Science\",\"volume\":\"12 \",\"pages\":\"1562879\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2025-04-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12042226/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in Veterinary Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.3389/fvets.2025.1562879\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Veterinary Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3389/fvets.2025.1562879","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Fermented Erigeron breviscapus flavonoids: anti-pseudorabies virus efficacy and mechanisms in vitro and in vivo.
Introduction: Erigeron breviscapus exhibits anti-inflammatory properties, protects neuronal cells and enhances immune function. Modern traditional Chinese medicine fermentation techniques can increase the bioactive compound content in Erigeron breviscapus. However, its potential therapeutic effects against the porcine pseudorabies virus (PRV) remain unclear.
Methods: A PRV infection model was established in mouse trigeminal ganglion (TG) cells to determine the optimal antiviral mode of action of flavonoids from fermented Erigeron breviscapus (FEBF). Additionally, a PRV-infected rat model was developed to evaluate the in vivo antiviral efficacy of FEBF.
Results: FEBF demonstrated a higher protective rate and a lower viral copy number compared to unfermented E. breviscapus flavonoids (EBF). The protective effect was most pronounced under toxicological and inhibitory conditions, surpassing the blocking effect. PRV infection upregulated TLR4, MyD88, and NF-κB p65 protein expression during the pre-infection phase, followed by their downregulation after 12 h. FEBF regulated PRV-induced changes in protein expression, restoring them to near-normal levels by 36 h. In vivo assessments of pathological injury, PRV viral load, neuronal count, and neuronal apoptosis indicated that FEBF provided superior neuroprotection compared to both Minocycline (MINO), a broad-spectrum neuroprotective drug, and unfermented EBF. Mechanistic studies further revealed that FEBF modulated microglial polarization and regulated the inflammatory cytokines IL-6, TNF-α, IL-4, and IL-10.
Conclusion: These findings demonstrate that FEBF exhibits significant antiviral effects against PRV in both in vitro and in vivo models. FEBF represents a promising candidate for the development of anti-PRV therapeutics.
期刊介绍:
Frontiers in Veterinary Science is a global, peer-reviewed, Open Access journal that bridges animal and human health, brings a comparative approach to medical and surgical challenges, and advances innovative biotechnology and therapy.
Veterinary research today is interdisciplinary, collaborative, and socially relevant, transforming how we understand and investigate animal health and disease. Fundamental research in emerging infectious diseases, predictive genomics, stem cell therapy, and translational modelling is grounded within the integrative social context of public and environmental health, wildlife conservation, novel biomarkers, societal well-being, and cutting-edge clinical practice and specialization. Frontiers in Veterinary Science brings a 21st-century approach—networked, collaborative, and Open Access—to communicate this progress and innovation to both the specialist and to the wider audience of readers in the field.
Frontiers in Veterinary Science publishes articles on outstanding discoveries across a wide spectrum of translational, foundational, and clinical research. The journal''s mission is to bring all relevant veterinary sciences together on a single platform with the goal of improving animal and human health.