运动聚类增强运动蛋白驱动的囊泡运输。

IF 3.2 3区 生物学 Q2 BIOPHYSICS
Biophysical journal Pub Date : 2025-06-17 Epub Date: 2025-05-05 DOI:10.1016/j.bpj.2025.04.033
Rui Jiang, Qingzhou Feng, Daguan Nong, You Jung Kang, David Sept, William O Hancock
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引用次数: 0

摘要

胞内囊泡通常由少量的动力蛋白和动力蛋白马达运输。然而,在体外生物物理研究中观察到的缓慢的微管结合率表明,长距离运输可能需要大量的马达。为了解决体内和体外研究中运动需求的差异,我们重建了由多个gfp标记的kinesin-1马达驱动的120纳米直径脂质体的运动。与基于先前结合率测量的预测一致,我们发现长距离运输需要大量的kinesin-1马达。我们假设体内观察的这种差异可能是由运动组织的差异引起的,并测试了运动聚类是否可以使用DNA支架提高运输效率。聚类三个马达增加了脂质体在大范围内的运动距离。我们的研究结果表明,与运动数量无关,运动在囊泡上的排列调节着运输距离,这表明运动组织的差异可能解释了体内和体外运动对远程运输需求的差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Motor clustering enhances kinesin-driven vesicle transport.

Intracellular vesicles are typically transported by a small number of kinesin and dynein motors. However, the slow microtubule binding rate of kinesin-1 observed in in vitro biophysical studies suggests that long-range transport may require a high number of motors. To address the discrepancy in motor requirements between in vivo and in vitro studies, we reconstituted motility of 120-nm-diameter liposomes driven by multiple GFP-labeled kinesin-1 motors. Consistent with predictions based on previous binding rate measurements, we found that long-distance transport requires a high number of kinesin-1 motors. We hypothesized that this discrepancy from in vivo observations may arise from differences in motor organization and tested whether motor clustering can enhance transport efficiency using a DNA scaffold. Clustering just three motors increased liposome travel distances across a wide range of motor numbers. Our findings demonstrate that, independent of motor number, the arrangement of motors on a vesicle regulates transport distance, suggesting that differences in motor organization may explain the disparity between in vivo and in vitro motor requirements for long-range transport.

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来源期刊
Biophysical journal
Biophysical journal 生物-生物物理
CiteScore
6.10
自引率
5.90%
发文量
3090
审稿时长
2 months
期刊介绍: BJ publishes original articles, letters, and perspectives on important problems in modern biophysics. The papers should be written so as to be of interest to a broad community of biophysicists. BJ welcomes experimental studies that employ quantitative physical approaches for the study of biological systems, including or spanning scales from molecule to whole organism. Experimental studies of a purely descriptive or phenomenological nature, with no theoretical or mechanistic underpinning, are not appropriate for publication in BJ. Theoretical studies should offer new insights into the understanding ofexperimental results or suggest new experimentally testable hypotheses. Articles reporting significant methodological or technological advances, which have potential to open new areas of biophysical investigation, are also suitable for publication in BJ. Papers describing improvements in accuracy or speed of existing methods or extra detail within methods described previously are not suitable for BJ.
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