多光子显微镜在微瓦级通过增益管理非线性放大和脉冲拾取。

IF 2.9 2区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Biomedical optics express Pub Date : 2025-03-27 eCollection Date: 2025-04-01 DOI:10.1364/BOE.557132

Katarzyna Kunio, Grzegorz Soboń, Jakub Bogusławski

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引用次数: 0

摘要

我们介绍了一种紧凑的全光纤激光系统，该系统具有增益管理非线性（GMN）放大Yb：光纤振荡器和集成脉冲拾取器。该系统提供39 fs脉冲，峰值功率为0.83 MW，脉冲重复率可调（0.3-15 MHz），可在非常低的激发功率（低至66 μ W）下实现多光子成像。其设计简化了集成，提高了实验的灵活性。兼容双光子和三光子激发，但也二次谐波产生显微镜，这个多功能系统提供了精确的控制成像参数，使其成为一个有效的工具，推进多光子显微镜和其他成像技术跨越各种实验环境。

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Multiphoton microscopy at a microwatt level via gain-managed nonlinear amplification and pulse-picking.

We introduce a compact, all-fiber laser system with a gain-managed nonlinear (GMN) amplified Yb:fiber oscillator and an integrated pulse-picker. The system delivers 39 fs pulses with peak powers of 0.83 MW and adjustable pulse repetition rates (0.3-15 MHz), enabling multiphoton imaging at remarkably low excitation powers (as low as 66 µW). Its design simplifies integration and enhances experimental flexibility. Compatible with two- and three-photon excitation, but also second harmonic generation microscopy, this versatile system offers precise control of imaging parameters, making it an effective tool for advancing multiphoton microscopy and other imaging techniques across various experimental environments.

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来源期刊

Biomedical optics express BIOCHEMICAL RESEARCH METHODS-OPTICS

CiteScore

6.80

自引率

11.80%

发文量

633

审稿时长

1 months

期刊介绍： The journal''s scope encompasses fundamental research, technology development, biomedical studies and clinical applications. BOEx focuses on the leading edge topics in the field, including: Tissue optics and spectroscopy Novel microscopies Optical coherence tomography Diffuse and fluorescence tomography Photoacoustic and multimodal imaging Molecular imaging and therapies Nanophotonic biosensing Optical biophysics/photobiology Microfluidic optical devices Vision research.