{"title":"氧化锌NPs通过增加pka的磷酸化来保护液体储存中的猪精子。","authors":"Yuanyou Li, Meiling He, Haohan Ran, Jie Wu, Jing Lv, Guangyu Liu, Yifan Wang, Zhongliang Jiang","doi":"10.1590/1984-3143-AR2024-0025","DOIUrl":null,"url":null,"abstract":"<p><p>It remains a problem to efficiently improve the boar sperm quality of liquid storage due to reactive oxygen species (ROS) accumulation. To reduce the effects of ROS on boar sperm, in this study, 1 μg/mL zinc oxide nanoparticles (ZnO NPs) was added into the extender of boar semen during liquid storage at 4°C and 17°C for 7 days. The finding revealed that sperm motility, viability, plasma membrane integrity (PMI) and acrosome integrity significantly increased when compared with the control group (<i>P</i> ˂ 0.05) Additionally, ZnO NPs significantly increased the levels of adenosine triphosphate (ATP), mitochondrial membrane potential (MMP), and antioxidation abilities (<i>P</i> ˂ 0.05) in boar sperm. Moreover, ZnO NPs could protect boar sperm from oxidative stress (OS) by inhibiting ROS-induced decrease of phosphorylation of PKA substrates (P-PKAs). Together, the current results suggest that ZnO NPs could be used as a novel antioxidant agent for semen preservation, which is helpful in improving the application of assisted reproductive technology in domestic animals.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 2","pages":"e20240025"},"PeriodicalIF":1.6000,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12020797/pdf/","citationCount":"0","resultStr":"{\"title\":\"ZnO NPs protect boar sperm in liquid storage through increasing the phosphorylation of PKAs.\",\"authors\":\"Yuanyou Li, Meiling He, Haohan Ran, Jie Wu, Jing Lv, Guangyu Liu, Yifan Wang, Zhongliang Jiang\",\"doi\":\"10.1590/1984-3143-AR2024-0025\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>It remains a problem to efficiently improve the boar sperm quality of liquid storage due to reactive oxygen species (ROS) accumulation. To reduce the effects of ROS on boar sperm, in this study, 1 μg/mL zinc oxide nanoparticles (ZnO NPs) was added into the extender of boar semen during liquid storage at 4°C and 17°C for 7 days. The finding revealed that sperm motility, viability, plasma membrane integrity (PMI) and acrosome integrity significantly increased when compared with the control group (<i>P</i> ˂ 0.05) Additionally, ZnO NPs significantly increased the levels of adenosine triphosphate (ATP), mitochondrial membrane potential (MMP), and antioxidation abilities (<i>P</i> ˂ 0.05) in boar sperm. Moreover, ZnO NPs could protect boar sperm from oxidative stress (OS) by inhibiting ROS-induced decrease of phosphorylation of PKA substrates (P-PKAs). Together, the current results suggest that ZnO NPs could be used as a novel antioxidant agent for semen preservation, which is helpful in improving the application of assisted reproductive technology in domestic animals.</p>\",\"PeriodicalId\":7889,\"journal\":{\"name\":\"Animal Reproduction\",\"volume\":\"22 2\",\"pages\":\"e20240025\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2025-04-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12020797/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Animal Reproduction\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1590/1984-3143-AR2024-0025\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Reproduction","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1590/1984-3143-AR2024-0025","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
ZnO NPs protect boar sperm in liquid storage through increasing the phosphorylation of PKAs.
It remains a problem to efficiently improve the boar sperm quality of liquid storage due to reactive oxygen species (ROS) accumulation. To reduce the effects of ROS on boar sperm, in this study, 1 μg/mL zinc oxide nanoparticles (ZnO NPs) was added into the extender of boar semen during liquid storage at 4°C and 17°C for 7 days. The finding revealed that sperm motility, viability, plasma membrane integrity (PMI) and acrosome integrity significantly increased when compared with the control group (P ˂ 0.05) Additionally, ZnO NPs significantly increased the levels of adenosine triphosphate (ATP), mitochondrial membrane potential (MMP), and antioxidation abilities (P ˂ 0.05) in boar sperm. Moreover, ZnO NPs could protect boar sperm from oxidative stress (OS) by inhibiting ROS-induced decrease of phosphorylation of PKA substrates (P-PKAs). Together, the current results suggest that ZnO NPs could be used as a novel antioxidant agent for semen preservation, which is helpful in improving the application of assisted reproductive technology in domestic animals.
期刊介绍：
Animal Reproduction (AR) publishes original scientific papers and invited literature reviews, in the form of Basic Research, Biotechnology, Applied Research and Review Articles, with the goal of contributing to a better understanding of phenomena related to animal reproduction.
The scope of the journal applies to students, researchers and practitioners in the fields of veterinary, biology and animal science, also being of interest to practitioners of human medicine. Animal Reproduction Journal is the official organ of the Brazilian College of Animal Reproduction in Brazil.
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