气体奋进:一种估算体外瘤胃发酵过程中甲烷动力学的创新设备。

IF 2.7 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Animals Pub Date : 2025-05-05 DOI:10.3390/ani15091331
Rashid Iqbal, Sheyla Arango, Franco Tagliapietra, Lucia Bailoni
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引用次数: 0

摘要

在提高饲料利用率的同时减少反刍动物甲烷排放的需求日益增长,这推动了创新体外测量技术的发展。这篇综述研究了Gas Endeavour (GES),这是一种自动化的体积测量仪器,可以实时量化瘤胃发酵过程中的总气体和甲烷产量。利用液体位移和浮力原理,GES集成了恒温控制水浴,专门的气体流动细胞和碱性二氧化碳吸收单元,以提供精确的发酵动力学数据。与通常依赖于人工测量和孵育后气相色谱的传统方法相比,GES提供连续监测和即时数据采集,减少了人工和潜在的错误。本文讨论了该系统的设计、操作挑战,如控制顶空压力和确保接种准备的一致性,以及它在动物营养和生物甲烷潜力评估中的应用。研究结果表明,通过进一步的标准化和方案完善,GES可以显著推进旨在优化反刍动物生产系统中饲料消化率和减少甲烷排放的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Gas Endeavour: An Innovative Equipment for Estimating Methane Kinetics During In Vitro Rumen Fermentation.

The growing need to reduce methane emissions from ruminants while enhancing feed utilization has driven the development of innovative in vitro measurement techniques. This review examines the Gas Endeavour (GES), an automated volumetric apparatus that quantifies both total gas and methane production in real time during rumen fermentation. Utilizing the principles of liquid displacement and buoyancy, the GES integrates a thermostatically controlled water bath, specialized gas flow cells, and an alkaline CO2 absorption unit to deliver precise kinetic data on fermentation. Compared to conventional methods-which often rely on manual measurements and post-incubation gas chromatography-the GES provides continuous monitoring and immediate data acquisition, reducing labour and potential errors. This review discusses the system's design, operational challenges such as controlling headspace pressure and ensuring consistent inoculum preparation, and its applications in both animal nutrition and biomethane potential assessments. The findings suggest that, with further standardization and protocol refinement, the GES could significantly advance research aimed at optimizing feed digestibility and mitigating methane emissions in ruminant production systems.

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来源期刊
Animals
Animals Agricultural and Biological Sciences-Animal Science and Zoology
CiteScore
4.90
自引率
16.70%
发文量
3015
审稿时长
20.52 days
期刊介绍: Animals (ISSN 2076-2615) is an international and interdisciplinary scholarly open access journal. It publishes original research articles, reviews, communications, and short notes that are relevant to any field of study that involves animals, including zoology, ethnozoology, animal science, animal ethics and animal welfare. However, preference will be given to those articles that provide an understanding of animals within a larger context (i.e., the animals'' interactions with the outside world, including humans). There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental details and/or method of study, must be provided for research articles. Articles submitted that involve subjecting animals to unnecessary pain or suffering will not be accepted, and all articles must be submitted with the necessary ethical approval (please refer to the Ethical Guidelines for more information).
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