评价4分钟4000g离心常规凝血检测方案。

IF 1.2 4区 医学 Q4 HEMATOLOGY
Sylvain Lamoine, Jean-Sébastien Blanchet, Aurélien Lebreton, Elodie Boissier
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引用次数: 0

摘要

离心是样品制备的关键步骤,占周转时间的重要组成部分。这一步对止血也很关键,因为止血需要低血小板计数才能产生可靠的结果。对于自动化实验室,离心可能是一个瓶颈,因此较短的离心时间将有利于管流和周转时间。在一个或两个离心循环后,我们在两个不同的中心比较了快速离心方案(4000g 4 min)和推荐方案(2200g 15 min)。评估每个方案对每一步血小板计数的影响,以验证该方案产生血小板贫血浆(PPP)的能力。比较了16个凝血参数的结果,验证了快速离心的可靠性。在一个中心,连续两循环离心对血小板计数进行了测试。与GEHT (Groupe Etude sur l’hsammostase et la Thrombose)方案相比,使用快速方案的单次离心循环产生的血浆中残留血小板增加。尽管存在这种差异,但凝血结果在两种方案之间是可互换的。此外,第二次离心循环产生的血浆平均残余血小板小于或等于10 × 109/l。单周期快速离心可用于评估凝血酶原时间(PT)、活化部分凝血活酶时间(aPTT)、aPTT高岭土、凝血酶时间(TT)、纤维蛋白原、抗凝血酶(AT)、d -二聚体、抗xa、因子II (FII)、因子V (FV)、因子VII (FVII)和因子X (FX)。对于冷冻血浆,应进行两次循环,然后进行第三次循环,以确保100%的样品含有少于10 × 109/l的血小板。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of a 4 min 4000g centrifugation protocol for routine coagulation assays.

Centrifugation is a critical step in sample preparation and accounts for an important part of turnaround time. This step is further critical for hemostasis, which requires a low platelet count to produce reliable results. For automated laboratories, centrifugation can represent a bottleneck and thus a shorter centrifugation time would benefit tube flow and turnaround time. We compared a rapid centrifugation protocol (4000g 4 min) with the recommended protocol (2200g 15 min) at two different centers, after one or two centrifugation cycles. The effect of each protocol was assessed on the platelet count at every step to verify the capacity of the protocol to yield platelet-poor plasma (PPP). Results on 16 coagulation parameters were compared to verify the reliability of rapid centrifugation. In one center, a consecutive two-cycle centrifugation had been tested on platelet count. A single centrifugation cycle, using the rapid protocol, produced plasma with increased residual platelets compared with the GEHT (Groupe Etude sur l'Hémostase et la Thrombose) protocol. Despite this difference, the coagulation results were interchangeable between the protocols. In addition, a second centrifugation cycle produces plasma with a mean residual platelet less than or equal to 10 × 109/l. A single cycle of rapid centrifugation can be used to assess prothrombin time (PT), activated partial thromboplastin time (aPTT), aPTT kaolin, thrombin time (TT), fibrinogen, antithrombin (AT), D-dimers, anti-Xa, factor II (FII), factor V (FV), factor VII (FVII), and factor X (FX). For frozen plasmas, a double-cycle followed by a third cycle should be performed to ensure that 100% of samples contain less than 10 × 109/l platelets.

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来源期刊
CiteScore
1.90
自引率
0.00%
发文量
111
审稿时长
4-8 weeks
期刊介绍: Blood Coagulation & Fibrinolysis is an international fully refereed journal that features review and original research articles on all clinical, laboratory and experimental aspects of haemostasis and thrombosis. The journal is devoted to publishing significant developments worldwide in the field of blood coagulation, fibrinolysis, thrombosis, platelets and the kininogen-kinin system, as well as dealing with those aspects of blood rheology relevant to haemostasis and the effects of drugs on haemostatic components
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