HIV-1整合酶的新细胞伴侣及其在病毒复制中的作用

IF 0.8 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Doklady Biochemistry and Biophysics Pub Date : 2025-05-11 DOI:10.1134/S1607672925600149

Y Y Agapkina, T Y Ponomareva, M V Vdovina, R H Ziganshin, A A Rozina, A N Anisenko, M B Gottikh

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引用次数: 0

摘要

细胞蛋白是病毒酶的伙伴，参与了人类免疫缺陷病毒I型（HIV-1）在不同阶段的复制。因此，参与病毒周期几个阶段的病毒酶整合酶与各种细胞蛋白相互作用。其中一些已经为人所知，一些的作用机制已经确立，但对整合酶的细胞伙伴的寻找仍在继续。在这项工作中，通过交联质谱（XL-MS）方法鉴定了HIV-1整合酶的细胞伴侣。已经确定了12个新的潜在整合酶伙伴，其中一些已经被检查了它们对HIV-1复制早期阶段的影响。

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New Cellular Partners of HIV-1 Integrase and their Role in Viral Replication.

Cellular proteins, partners of viral enzymes, are involved in the replication of human immunodeficiency virus type I (HIV-1) at various stages. Thus, the viral enzyme integrase, participating in several stages of the viral cycle, interacts with various cellular proteins. A number of them are already known, and for some the mechanism of action has been established, but the search for cellular partners of integrase continues. In this work, the identification of cellular partners of HIV-1 integrase has been carried out by the method of cross-linking followed by mass spectrometry (XL-MS). Twelve new potential integrase partners have been identified, and some of them have been examined for their effect on the early stages of HIV-1 replication.

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来源期刊

Doklady Biochemistry and Biophysics 生物-生化与分子生物学

CiteScore

1.60

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.