{"title":"NT-OmpA生物乳化剂：结构和多糖介导的乳化性能增强。","authors":"Naeema Mohseni Sani, Mahbubeh Talaee, Fatemeh Saadati, Faranak Ashoori, Javad Zamani, Hossein Shahbani Zahiri, Hojatollah Vali, Kambiz Akbari Noghabi","doi":"10.1002/cplu.202400774","DOIUrl":null,"url":null,"abstract":"<p><p>Our recent studies found that NT-OmpA (N-terminally truncated SA01-OmpA) has distinct advantages over full-length SA01-OmpA, including non-cytotoxicity, enhanced emulsifying capacity, and higher production yield. This study builds upon prior research by investigating the emulsifying properties of NT-OmpA under various environmental conditions, such as temperature, salinity, and pH. Molecular dynamics (MD) simulations and circular dichroism (CD) spectroscopy revealed significant alterations in the secondary structure of NT-OmpA under extreme conditions, specifically at alkaline pH (pH 11) and elevated temperatures (90°C). Circular dichroism (CD) spectroscopy showed a significant decrease in alpha-helical content and increased beta-sheet structures, validating NT-OmpA's pH stability. We investigated NT-OmpA's effectiveness when combined with various polysaccharides to enhance its functional properties. Dextran (emulsification index of 76.94 ± 0.05 %) exhibited superior emulsifying activity compared to glucose, agarose, starch, and NT-OmpA alone. NT-OmpA's emulsifying characteristics are considerably improved by dextran, making this combination a promising next-generation emulsifier. These findings underscore the structural resilience and functional adaptability of NT-OmpA, especially in challenging environmental conditions, and highlight its potential in industrial applications requiring robust emulsifying agents.</p>","PeriodicalId":148,"journal":{"name":"ChemPlusChem","volume":" ","pages":"e202400774"},"PeriodicalIF":3.0000,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"NT-OmpA Bioemulsifier: Structural Insights and Polysaccharide-Mediated Enhancement of Its Emulsifying Performance.\",\"authors\":\"Naeema Mohseni Sani, Mahbubeh Talaee, Fatemeh Saadati, Faranak Ashoori, Javad Zamani, Hossein Shahbani Zahiri, Hojatollah Vali, Kambiz Akbari Noghabi\",\"doi\":\"10.1002/cplu.202400774\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Our recent studies found that NT-OmpA (N-terminally truncated SA01-OmpA) has distinct advantages over full-length SA01-OmpA, including non-cytotoxicity, enhanced emulsifying capacity, and higher production yield. This study builds upon prior research by investigating the emulsifying properties of NT-OmpA under various environmental conditions, such as temperature, salinity, and pH. Molecular dynamics (MD) simulations and circular dichroism (CD) spectroscopy revealed significant alterations in the secondary structure of NT-OmpA under extreme conditions, specifically at alkaline pH (pH 11) and elevated temperatures (90°C). Circular dichroism (CD) spectroscopy showed a significant decrease in alpha-helical content and increased beta-sheet structures, validating NT-OmpA's pH stability. We investigated NT-OmpA's effectiveness when combined with various polysaccharides to enhance its functional properties. Dextran (emulsification index of 76.94 ± 0.05 %) exhibited superior emulsifying activity compared to glucose, agarose, starch, and NT-OmpA alone. NT-OmpA's emulsifying characteristics are considerably improved by dextran, making this combination a promising next-generation emulsifier. These findings underscore the structural resilience and functional adaptability of NT-OmpA, especially in challenging environmental conditions, and highlight its potential in industrial applications requiring robust emulsifying agents.</p>\",\"PeriodicalId\":148,\"journal\":{\"name\":\"ChemPlusChem\",\"volume\":\" \",\"pages\":\"e202400774\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2025-04-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ChemPlusChem\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1002/cplu.202400774\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ChemPlusChem","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1002/cplu.202400774","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
NT-OmpA Bioemulsifier: Structural Insights and Polysaccharide-Mediated Enhancement of Its Emulsifying Performance.
Our recent studies found that NT-OmpA (N-terminally truncated SA01-OmpA) has distinct advantages over full-length SA01-OmpA, including non-cytotoxicity, enhanced emulsifying capacity, and higher production yield. This study builds upon prior research by investigating the emulsifying properties of NT-OmpA under various environmental conditions, such as temperature, salinity, and pH. Molecular dynamics (MD) simulations and circular dichroism (CD) spectroscopy revealed significant alterations in the secondary structure of NT-OmpA under extreme conditions, specifically at alkaline pH (pH 11) and elevated temperatures (90°C). Circular dichroism (CD) spectroscopy showed a significant decrease in alpha-helical content and increased beta-sheet structures, validating NT-OmpA's pH stability. We investigated NT-OmpA's effectiveness when combined with various polysaccharides to enhance its functional properties. Dextran (emulsification index of 76.94 ± 0.05 %) exhibited superior emulsifying activity compared to glucose, agarose, starch, and NT-OmpA alone. NT-OmpA's emulsifying characteristics are considerably improved by dextran, making this combination a promising next-generation emulsifier. These findings underscore the structural resilience and functional adaptability of NT-OmpA, especially in challenging environmental conditions, and highlight its potential in industrial applications requiring robust emulsifying agents.
期刊介绍：
ChemPlusChem is a peer-reviewed, general chemistry journal that brings readers the very best in multidisciplinary research centering on chemistry. It is published on behalf of Chemistry Europe, an association of 16 European chemical societies.
Fully comprehensive in its scope, ChemPlusChem publishes articles covering new results from at least two different aspects (subfields) of chemistry or one of chemistry and one of another scientific discipline (one chemistry topic plus another one, hence the title ChemPlusChem). All suitable submissions undergo balanced peer review by experts in the field to ensure the highest quality, originality, relevance, significance, and validity.
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