余叶甲醇提取物对HCT-116结肠癌细胞株的抗癌作用。

Q3 Veterinary
Archives of Razi Institute Pub Date : 2024-10-31 eCollection Date: 2024-10-01 DOI:10.32592/ARI.2024.79.5.1013
K Y Hiremath, S Shinde, U K Jambale, A P Paralashetter, B N Anusha, A Betageri, V Agnihotri, S S Harindral, S Y Mesta, C T Shivasharana
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引用次数: 0

摘要

叶兰是大戟科的一种植物。这种植物原产于印度、孟加拉国、中国和马来群岛的热带地区。这种植物以其药用特性而闻名,传统上被用作利尿剂、冷却药,以及治疗海绵和出血的牙龈。文献显示该植物具有保肝、降血脂、抗伤、镇痛、抗炎、抗疟、细胞毒、抗菌、抗癌等特性。一种具有显著药用潜力的植物及其叶片被研究用于治疗糖尿病和腹泻的潜在应用。本研究通过植物化学分析和GC-MS分析,重点分析了网柳甲醇叶提取物的化学特征,揭示了含有活性化学成分的9个主要峰。FTIR分析显示存在6个生物活性官能团。甲醇叶提取物中酚类含量为93.44 mg/g,类黄酮含量为55.35 mg/g。植物叶片的元素浓度显示了12种元素的存在。DPPH和PM实验显示了叶提取物的抗氧化作用,叶提取物对HCT-116结肠癌细胞株24 h的细胞生长抑制率为28.56%,且呈剂量依赖性。本文首次报道了叶提取物对结肠癌的光谱学和抗癌作用。本研究为进一步研究余叶生物活性成分及其作用机制开辟了道路,为今后的研究和潜在的药物开发铺平了道路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anticancer effect of Phyllanthus reticulatus methanolic leaf extract on HCT-116 colon cancer cell line using GC-MS and FTIR analysis.

Phyllanthus reticulatus is a plant species belonging to the family Euphorbiaceae. The plant is native to tropical areas of India, Bangladesh, China, and the Malay Islands. The plant is known for its medicinal properties and has been traditionally used as a diuretic, cooling medicine, and remedy for spongy and bleeding gums. Literature revealed the plant's hepatoprotective, hypolipidemic, antinociceptive, analgesic, anti-inflammatory, antimalarial, cytotoxic, and antimicrobial, anticancer properties. A plant species with significant medicinal potential and its leaves have been studied for their potential therapeutic applications in managing diabetes and diarrhea. This study highlights the chemical profile of P. reticulatus methanolic leaf extract by phytochemical and GC-MS analysis, revealing nine major peaks with active chemical components. FTIR analysis showed the presence of six biologically active functional groups. The methanolic leaf extract showed the existence of 93.44 mg/g, a significant amount of phenolic content, and 55.35 mg/g of flavonoid content. The elemental concentration of plant leaf revealed the presence of 12 elements. The DPPH and PM assay showed the antioxidant properties of the leaf extract, as evidenced by its anticancer property of the leaf extract with 28.56 % cell growth inhibition on the HCT-116 colon cancer cell line at 24 h in a dose-dependent manner. This is the first report on Phyllanthus reticulatus, which reports the spectroscopic and anticancer properties of the leaf extract on colon cancer. The study opens avenues for further investigation into the bioactive constituents of Phyllanthus reticulatus leaves and their mechanisms of action, paving the way for future studies and potential drug development.

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来源期刊
Archives of Razi Institute
Archives of Razi Institute Veterinary-Veterinary (all)
CiteScore
1.50
自引率
0.00%
发文量
108
审稿时长
12 weeks
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