抗多药耐药细菌感染的噬菌体基因组工程创新。

IF 1.9 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY
Riska Ayu Febrianti, Erlia Narulita, Erma Sulistyaningsih, Hardian Susilo Addy
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引用次数: 0

摘要

噬菌体工程是解决多药耐药(MDR)细菌感染的一种很有前途的策略,这种细菌感染由于抗生素的过度使用而对公共卫生构成重大挑战。细菌可以产生抗性机制,如受体修饰和抗病毒防御系统的激活,这进一步复杂化了噬菌体治疗的应用。此外,长期噬菌体治疗可导致抗噬菌体抗体的产生,这可能会干扰治疗。这些因素需要先进的工程技术来提高噬菌体的功效并扩大其宿主范围。基因组工程方法的最新进展,包括CRISPR/Cas9、同源重组和其他合成生物学技术,为这些挑战提供了有希望的解决方案。通过修饰受体结合蛋白和使用高产筛选方法,研究人员可以创造出更好的噬菌体来有效地靶向耐多药耐药细菌。此外,了解噬菌体与其细菌宿主之间复杂的相互作用对于指导这些工程工作至关重要。未来的发展前景在于将这些先进的工程技术整合到临床实践中,有可能将噬菌体置于对抗耐多药细菌感染的前沿。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Innovations in Bacteriophage Genome Engineering for Combating Multidrug-Resistant Bacterial Infections.

Bacteriophage engineering is a promising strategy to address multidrug-resistant (MDR) bacterial infections that pose significant challenges to public health due to the overuse of antibiotics. Bacteria can develop resistance mechanisms, such as receptor modification and activation of antiviral defense systems, which further complicates the application of phage therapy. Additionally, long-term phage therapy can result in the production of anti-phage antibodies, which may interfere with treatment. These factors require advanced engineering techniques to improve the efficacy of phages and expand their host range. Recent advances in genome engineering methods, including CRISPR/Cas9, homologous recombination, and other synthetic biology techniques, offer promising solutions to these challenges. By modifying receptor-binding proteins and using high-yield screening methods, researchers can create phages that are better equipped to target MDR bacteria effectively. Furthermore, understanding the intricate interactions between phages and their bacterial hosts is critical to guiding these engineering efforts. Future development perspectives lie in integrating these advanced engineering techniques into clinical practice, potentially putting bacteriophages at the forefront of fighting MDR bacterial infections.

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来源期刊
Foodborne pathogens and disease
Foodborne pathogens and disease 医学-食品科技
CiteScore
5.30
自引率
3.60%
发文量
80
审稿时长
1 months
期刊介绍: Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.
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