营养物质的富集改变了美洲牛蛙体内的微生物群,增加了壶菌负荷。

IF 1.1 4区 农林科学 Q3 FISHERIES
Katie Pincus, Patrick J Kearns, Kaitlyn Williams, Douglas C Woodhams
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引用次数: 0

摘要

农业实践对流域动态、水质和水生生物的健康有着深远的影响。一个主要问题是农业污染,特别是营养过剩,这可能会增加各种宿主-病原体关系中的疾病风险。然而,驱动这种效应的确切机制仍然不确定。据信,营养水平升高会显著影响水生环境细菌种群,可能重塑水生生物的微生物群,并影响它们对疾病的易感性。尽管如此,营养富集对宿主微生物群的影响作为水生生物疾病的联系在很大程度上被忽视了。在本研究中,我们研究了营养物质的富集对美洲牛蛙皮肤相关微生物群落的影响。与对照池相比,富营养池的细菌丰富度和群落组成发生了显著变化。虽然群落对树突壶菌(Batrachochytrium dendroatidis, Bd)的抑制比例没有显著变化,但富营养化池塘的Bd负荷明显较高。根据Biolog EcoPlates测量,营养物的富集显著改变了碳利用模式,并且抗生素耐药性在所有池塘和样本中普遍存在,营养物富集的池塘对甲氧苄氨嘧啶、磺胺甲基嗪和氯霉素的耐药性明显更高。我们的研究结果表明,营养富集影响美国牛蛙皮肤相关微生物群落的结构和功能,影响Bd负荷和抗生素耐药性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Nutrient enrichment alters the microbiome and increases chytrid load in the American bullfrog Lithobates catesbeianus.

Agricultural practices have a profound impact on watershed dynamics, water quality, and the well-being of aquatic life. One major concern is agricultural pollution, particularly the excess of nutrients, which can elevate disease risks in various host-pathogen relationships. However, the exact mechanisms driving this effect remain uncertain. Elevated nutrient levels are believed to significantly influence populations of aquatic environmental bacteria, potentially reshaping the microbiomes of aquatic organisms and affecting their vulnerability to disease. Despite this, the impact of nutrient enrichment on host microbiomes as a link to diseases in aquatic organisms has been largely overlooked. In this study, we investigated the impact of nutrient enrichment on the skin-associated microbial communities of the American bullfrog Lithobates catesbeianus. We observed a significant shift in bacterial richness and community composition in nutrient-enriched ponds compared with reference ponds. Although the proportion of the community inhibitory towards Batrachochytrium dendrobatidis (Bd) did not change significantly, Bd loads were markedly higher in nutrient-enriched ponds. Nutrient enrichment significantly altered carbon utilization patterns as measured by Biolog EcoPlates, and antibiotic resistance was prevalent across all ponds and samples, with resistance to trimethoprim, sulfamethazine, and chloramphenicol significantly higher in nutrient-enriched ponds. Our findings indicate that nutrient enrichment affects the structure and function of skin-associated microbial communities in American bullfrogs, influencing both Bd load and antibiotic resistance.

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来源期刊
Diseases of aquatic organisms
Diseases of aquatic organisms 农林科学-兽医学
CiteScore
3.10
自引率
0.00%
发文量
53
审稿时长
8-16 weeks
期刊介绍: DAO publishes Research Articles, Reviews, and Notes, as well as Comments/Reply Comments (for details see DAO 48:161), Theme Sections and Opinion Pieces. For details consult the Guidelines for Authors. Papers may cover all forms of life - animals, plants and microorganisms - in marine, limnetic and brackish habitats. DAO''s scope includes any research focusing on diseases in aquatic organisms, specifically: -Diseases caused by coexisting organisms, e.g. viruses, bacteria, fungi, protistans, metazoans; characterization of pathogens -Diseases caused by abiotic factors (critical intensities of environmental properties, including pollution)- Diseases due to internal circumstances (innate, idiopathic, genetic)- Diseases due to proliferative disorders (neoplasms)- Disease diagnosis, treatment and prevention- Molecular aspects of diseases- Nutritional disorders- Stress and physical injuries- Epidemiology/epizootiology- Parasitology- Toxicology- Diseases of aquatic organisms affecting human health and well-being (with the focus on the aquatic organism)- Diseases as indicators of humanity''s detrimental impact on nature- Genomics, proteomics and metabolomics of disease- Immunology and disease prevention- Animal welfare- Zoonosis
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