{"title":"用于动物源性食品中氨苄西林残留视觉检测的智能手机集成比色传感器。","authors":"Reena K Sajwan, Pratima R Solanki","doi":"10.1016/j.talanta.2025.128057","DOIUrl":null,"url":null,"abstract":"<p><p>The antibiotic residue in food accelerates bacteria's resistance process that is responsible for serious health issues. A smartphone-integrated ratiometric colorimetric aptasensor has been developed for ampicillin (AMP) detection in milk and egg samples. The sensing phenomenon utilizes the optical and color-changing properties of gold nanoparticles (AuNPs) and specific aptamer with higher affinity towards AMP for its selective detection. The aptasensor shows linear response with AMP concentration 0.06-9.9 μM with a lower detection limit (LOD) of 45.0 nM. The smartphone-integrated color analyzer application was used to identify the solution's red, green, and blue (RGB) color intensity and calculate the G/R ratio of color intensities in the presence of AMP. Aptasensor show linear response with AMP concentration (0-6.6 μM), with a LOD of 53.0 nM. The sensor's results were further validated with liquid chromatography and mass spectroscopy techniques (LC-MS), showing that the developed sensor is suitable for food monitoring and safety applications.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"128057"},"PeriodicalIF":5.6000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Smartphone-integrated ratiometric colorimetric aptasensor for visual detection of ampicillin residue in animal-derived food samples.\",\"authors\":\"Reena K Sajwan, Pratima R Solanki\",\"doi\":\"10.1016/j.talanta.2025.128057\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The antibiotic residue in food accelerates bacteria's resistance process that is responsible for serious health issues. A smartphone-integrated ratiometric colorimetric aptasensor has been developed for ampicillin (AMP) detection in milk and egg samples. The sensing phenomenon utilizes the optical and color-changing properties of gold nanoparticles (AuNPs) and specific aptamer with higher affinity towards AMP for its selective detection. The aptasensor shows linear response with AMP concentration 0.06-9.9 μM with a lower detection limit (LOD) of 45.0 nM. The smartphone-integrated color analyzer application was used to identify the solution's red, green, and blue (RGB) color intensity and calculate the G/R ratio of color intensities in the presence of AMP. Aptasensor show linear response with AMP concentration (0-6.6 μM), with a LOD of 53.0 nM. The sensor's results were further validated with liquid chromatography and mass spectroscopy techniques (LC-MS), showing that the developed sensor is suitable for food monitoring and safety applications.</p>\",\"PeriodicalId\":435,\"journal\":{\"name\":\"Talanta\",\"volume\":\"293 \",\"pages\":\"128057\"},\"PeriodicalIF\":5.6000,\"publicationDate\":\"2025-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Talanta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1016/j.talanta.2025.128057\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/4/2 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Talanta","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.talanta.2025.128057","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/4/2 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Smartphone-integrated ratiometric colorimetric aptasensor for visual detection of ampicillin residue in animal-derived food samples.
The antibiotic residue in food accelerates bacteria's resistance process that is responsible for serious health issues. A smartphone-integrated ratiometric colorimetric aptasensor has been developed for ampicillin (AMP) detection in milk and egg samples. The sensing phenomenon utilizes the optical and color-changing properties of gold nanoparticles (AuNPs) and specific aptamer with higher affinity towards AMP for its selective detection. The aptasensor shows linear response with AMP concentration 0.06-9.9 μM with a lower detection limit (LOD) of 45.0 nM. The smartphone-integrated color analyzer application was used to identify the solution's red, green, and blue (RGB) color intensity and calculate the G/R ratio of color intensities in the presence of AMP. Aptasensor show linear response with AMP concentration (0-6.6 μM), with a LOD of 53.0 nM. The sensor's results were further validated with liquid chromatography and mass spectroscopy techniques (LC-MS), showing that the developed sensor is suitable for food monitoring and safety applications.
期刊介绍：
Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome.
Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.
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