Huiping Jia, Wenwen Chu, Dong Zhang, Kai Li, Wenpu Huang, Xiaoyun Li
{"title":"新疆濒危中蒙海狸(Castor fiber birulai)寄生物Travassosius rufus Khalil, 1922的形态、分子特征和系统发育(圆圆纲:毛圆纲科)。","authors":"Huiping Jia, Wenwen Chu, Dong Zhang, Kai Li, Wenpu Huang, Xiaoyun Li","doi":"10.3390/ani15091339","DOIUrl":null,"url":null,"abstract":"<p><p>The genus <i>Travassosius</i> Khalil, 1922, the smallest genus in the subfamily Trichostrongylinae (family Trichostrongylidae), primarily infects the only two extant beaver species worldwide and can be lethal in severe infections. However, the mitochondrial genome evolution of <i>Travassosius</i> remains poorly understood, and its phylogenetic placement within Trichostrongylinae is still unresolved. In this study, we applied both morphological techniques (differential interference contrast microscopy) and molecular tools (nuclear ITS2 and mitochondrial genome) to examine <i>T. rufus</i> Khalil, 1922. Specimens were collected from the Sino-Mongolian beaver, a subspecies of the Eurasian beaver native to the Ulungur River Basin in northern Xinjiang, China. This work presents the first complete mitochondrial genome sequence and annotation of <i>T. rufus</i>, and it is also the first mitochondrial genome reported for the genus <i>Travassosius</i>. The mitochondrial genome of <i>T. rufus</i> measures 13,646 bp and contains 36 genes, including 12 protein-coding genes (PCGs) (excluding <i>atp</i>8), 22 transfer RNA genes, and 2 ribosomal RNA genes. Phylogenetic analysis based on amino acid sequences of 12 mitochondrial PCGs strongly supports the distinctiveness of the genus <i>Travassosius</i>. Additionally, <i>T. rufus</i> appears to be closely related to <i>Nematodirus</i> within Trichostrongylinae. This study also addresses the possible consequences of parasitic infection for the Sino-Mongolian beaver and offers a scientific foundation for conserving this endangered subspecies and managing parasitic diseases in its population.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 9","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12071037/pdf/","citationCount":"0","resultStr":"{\"title\":\"Morphology, Molecular Characterization, and Phylogeny of <i>Travassosius rufus</i> Khalil, 1922 (Strongylidea: Trichostrongylidae), a Parasite from Endangered Sino-Mongolian Beaver (<i>Castor fiber birulai</i>) in Xinjiang, China.\",\"authors\":\"Huiping Jia, Wenwen Chu, Dong Zhang, Kai Li, Wenpu Huang, Xiaoyun Li\",\"doi\":\"10.3390/ani15091339\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The genus <i>Travassosius</i> Khalil, 1922, the smallest genus in the subfamily Trichostrongylinae (family Trichostrongylidae), primarily infects the only two extant beaver species worldwide and can be lethal in severe infections. However, the mitochondrial genome evolution of <i>Travassosius</i> remains poorly understood, and its phylogenetic placement within Trichostrongylinae is still unresolved. In this study, we applied both morphological techniques (differential interference contrast microscopy) and molecular tools (nuclear ITS2 and mitochondrial genome) to examine <i>T. rufus</i> Khalil, 1922. Specimens were collected from the Sino-Mongolian beaver, a subspecies of the Eurasian beaver native to the Ulungur River Basin in northern Xinjiang, China. This work presents the first complete mitochondrial genome sequence and annotation of <i>T. rufus</i>, and it is also the first mitochondrial genome reported for the genus <i>Travassosius</i>. The mitochondrial genome of <i>T. rufus</i> measures 13,646 bp and contains 36 genes, including 12 protein-coding genes (PCGs) (excluding <i>atp</i>8), 22 transfer RNA genes, and 2 ribosomal RNA genes. Phylogenetic analysis based on amino acid sequences of 12 mitochondrial PCGs strongly supports the distinctiveness of the genus <i>Travassosius</i>. Additionally, <i>T. rufus</i> appears to be closely related to <i>Nematodirus</i> within Trichostrongylinae. This study also addresses the possible consequences of parasitic infection for the Sino-Mongolian beaver and offers a scientific foundation for conserving this endangered subspecies and managing parasitic diseases in its population.</p>\",\"PeriodicalId\":7955,\"journal\":{\"name\":\"Animals\",\"volume\":\"15 9\",\"pages\":\"\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2025-05-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12071037/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Animals\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.3390/ani15091339\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animals","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3390/ani15091339","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Morphology, Molecular Characterization, and Phylogeny of Travassosius rufus Khalil, 1922 (Strongylidea: Trichostrongylidae), a Parasite from Endangered Sino-Mongolian Beaver (Castor fiber birulai) in Xinjiang, China.
The genus Travassosius Khalil, 1922, the smallest genus in the subfamily Trichostrongylinae (family Trichostrongylidae), primarily infects the only two extant beaver species worldwide and can be lethal in severe infections. However, the mitochondrial genome evolution of Travassosius remains poorly understood, and its phylogenetic placement within Trichostrongylinae is still unresolved. In this study, we applied both morphological techniques (differential interference contrast microscopy) and molecular tools (nuclear ITS2 and mitochondrial genome) to examine T. rufus Khalil, 1922. Specimens were collected from the Sino-Mongolian beaver, a subspecies of the Eurasian beaver native to the Ulungur River Basin in northern Xinjiang, China. This work presents the first complete mitochondrial genome sequence and annotation of T. rufus, and it is also the first mitochondrial genome reported for the genus Travassosius. The mitochondrial genome of T. rufus measures 13,646 bp and contains 36 genes, including 12 protein-coding genes (PCGs) (excluding atp8), 22 transfer RNA genes, and 2 ribosomal RNA genes. Phylogenetic analysis based on amino acid sequences of 12 mitochondrial PCGs strongly supports the distinctiveness of the genus Travassosius. Additionally, T. rufus appears to be closely related to Nematodirus within Trichostrongylinae. This study also addresses the possible consequences of parasitic infection for the Sino-Mongolian beaver and offers a scientific foundation for conserving this endangered subspecies and managing parasitic diseases in its population.
AnimalsAgricultural and Biological Sciences-Animal Science and Zoology
CiteScore
4.90
自引率
16.70%
发文量
3015
审稿时长
20.52 days
期刊介绍:
Animals (ISSN 2076-2615) is an international and interdisciplinary scholarly open access journal. It publishes original research articles, reviews, communications, and short notes that are relevant to any field of study that involves animals, including zoology, ethnozoology, animal science, animal ethics and animal welfare. However, preference will be given to those articles that provide an understanding of animals within a larger context (i.e., the animals'' interactions with the outside world, including humans). There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental details and/or method of study, must be provided for research articles. Articles submitted that involve subjecting animals to unnecessary pain or suffering will not be accepted, and all articles must be submitted with the necessary ethical approval (please refer to the Ethical Guidelines for more information).