新疆濒危中蒙海狸(Castor fiber birulai)寄生物Travassosius rufus Khalil, 1922的形态、分子特征和系统发育(圆圆纲:毛圆纲科)。

IF 2.7 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Animals Pub Date : 2025-05-06 DOI:10.3390/ani15091339
Huiping Jia, Wenwen Chu, Dong Zhang, Kai Li, Wenpu Huang, Xiaoyun Li
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引用次数: 0

摘要

Travassosius Khalil属,1922年,是毛圆线虫亚科(毛圆线虫科)中最小的属,主要感染世界上仅存的两种海狸,严重感染可致命。然而,Travassosius的线粒体基因组进化仍然知之甚少,其在毛线虫科中的系统发育位置仍然没有得到解决。在这项研究中,我们应用形态学技术(差示干涉对比显微镜)和分子工具(核ITS2和线粒体基因组)对T. rufus Khalil, 1922进行了研究。本文采集了原产于新疆北部乌伦古尔河流域的欧亚海狸亚种中蒙海狸的标本。本研究首次报道了T. rufus线粒体基因组的完整序列和注释,也是首次报道的Travassosius属线粒体基因组。T. rufus线粒体基因组全长13646 bp,包含36个基因,包括12个蛋白质编码基因(PCGs)(不包括atp8)、22个转移RNA基因和2个核糖体RNA基因。基于12个线粒体PCGs氨基酸序列的系统发育分析有力地支持了travassous属的独特性。此外,T. rufus似乎与毛线虫科内的线虫密切相关。本研究还探讨了寄生虫感染对中蒙海狸可能造成的后果,为保护中蒙海狸这一濒危亚种和管理其种群中的寄生虫疾病提供了科学依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Morphology, Molecular Characterization, and Phylogeny of Travassosius rufus Khalil, 1922 (Strongylidea: Trichostrongylidae), a Parasite from Endangered Sino-Mongolian Beaver (Castor fiber birulai) in Xinjiang, China.

The genus Travassosius Khalil, 1922, the smallest genus in the subfamily Trichostrongylinae (family Trichostrongylidae), primarily infects the only two extant beaver species worldwide and can be lethal in severe infections. However, the mitochondrial genome evolution of Travassosius remains poorly understood, and its phylogenetic placement within Trichostrongylinae is still unresolved. In this study, we applied both morphological techniques (differential interference contrast microscopy) and molecular tools (nuclear ITS2 and mitochondrial genome) to examine T. rufus Khalil, 1922. Specimens were collected from the Sino-Mongolian beaver, a subspecies of the Eurasian beaver native to the Ulungur River Basin in northern Xinjiang, China. This work presents the first complete mitochondrial genome sequence and annotation of T. rufus, and it is also the first mitochondrial genome reported for the genus Travassosius. The mitochondrial genome of T. rufus measures 13,646 bp and contains 36 genes, including 12 protein-coding genes (PCGs) (excluding atp8), 22 transfer RNA genes, and 2 ribosomal RNA genes. Phylogenetic analysis based on amino acid sequences of 12 mitochondrial PCGs strongly supports the distinctiveness of the genus Travassosius. Additionally, T. rufus appears to be closely related to Nematodirus within Trichostrongylinae. This study also addresses the possible consequences of parasitic infection for the Sino-Mongolian beaver and offers a scientific foundation for conserving this endangered subspecies and managing parasitic diseases in its population.

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来源期刊
Animals
Animals Agricultural and Biological Sciences-Animal Science and Zoology
CiteScore
4.90
自引率
16.70%
发文量
3015
审稿时长
20.52 days
期刊介绍: Animals (ISSN 2076-2615) is an international and interdisciplinary scholarly open access journal. It publishes original research articles, reviews, communications, and short notes that are relevant to any field of study that involves animals, including zoology, ethnozoology, animal science, animal ethics and animal welfare. However, preference will be given to those articles that provide an understanding of animals within a larger context (i.e., the animals'' interactions with the outside world, including humans). There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental details and/or method of study, must be provided for research articles. Articles submitted that involve subjecting animals to unnecessary pain or suffering will not be accepted, and all articles must be submitted with the necessary ethical approval (please refer to the Ethical Guidelines for more information).
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