{"title":"肺结核患者外周血淋巴细胞腺苷脱氨酶水平的诊断准确性。","authors":"Ashish Anantrao Jadhav, Ashok Kumar, Alkesh Kumar Khurana, Jagat Rakesh Kanwar, Ashwin Kotnis, Suryabhan Lahanu Lokhande","doi":"10.4103/aam.aam_212_24","DOIUrl":null,"url":null,"abstract":"<p><strong>Context: </strong>Tuberculosis (TB) is a well-known global health problem with one-fifth of the global burden in India. Although there are diagnostic and prognostic markers for TB, there is lack of specificity and sensitivity of diagnostic methods.</p><p><strong>Aims: </strong>The goal of this study was to assess the diagnostic value of blood lymphocyte adenosine deaminase (ADA) levels in pulmonary TB patients.</p><p><strong>Subjects and methods: </strong>This was a prospective observational case-control study. A total of 80 subjects were included in this study and were divided into two groups: 40 people who were healthy in every respect and 40 patients with pulmonary TB. ADA activity was measured in each group by the Giusti and Galanti method using a commercial kit.</p><p><strong>Statistical analysis used: </strong>Data were analyzed using t-test with receiver operating characteristic curve analysis done to identify the cutoff values for ADA that had maximum sensitivity and specificity.</p><p><strong>Results: </strong>The mean serum ADA level in the pulmonary TB group was significantly increased as compared to control group (P < 0.0001), whereas blood lymphocyte ADA levels as well as blood lymphocyte/serum ADA ratio in the pulmonary TB group were significantly decreased as compared control group (P < 0.0001). In regard to the cutoff value of <34.06 IU/mg protein in patients with pulmonary TB, sensitivity and specificity were 90.00% and 80.00%, respectively.</p><p><strong>Conclusions: </strong>Blood lymphocyte ADA level may be used as a biomarker for the diagnosis of pulmonary TB.</p>","PeriodicalId":7938,"journal":{"name":"Annals of African Medicine","volume":" ","pages":""},"PeriodicalIF":0.6000,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Diagnostic Accuracy of Adenosine Deaminase Levels in Peripheral Blood Lymphocyte of Patients with Pulmonary Tuberculosis.\",\"authors\":\"Ashish Anantrao Jadhav, Ashok Kumar, Alkesh Kumar Khurana, Jagat Rakesh Kanwar, Ashwin Kotnis, Suryabhan Lahanu Lokhande\",\"doi\":\"10.4103/aam.aam_212_24\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Context: </strong>Tuberculosis (TB) is a well-known global health problem with one-fifth of the global burden in India. Although there are diagnostic and prognostic markers for TB, there is lack of specificity and sensitivity of diagnostic methods.</p><p><strong>Aims: </strong>The goal of this study was to assess the diagnostic value of blood lymphocyte adenosine deaminase (ADA) levels in pulmonary TB patients.</p><p><strong>Subjects and methods: </strong>This was a prospective observational case-control study. A total of 80 subjects were included in this study and were divided into two groups: 40 people who were healthy in every respect and 40 patients with pulmonary TB. ADA activity was measured in each group by the Giusti and Galanti method using a commercial kit.</p><p><strong>Statistical analysis used: </strong>Data were analyzed using t-test with receiver operating characteristic curve analysis done to identify the cutoff values for ADA that had maximum sensitivity and specificity.</p><p><strong>Results: </strong>The mean serum ADA level in the pulmonary TB group was significantly increased as compared to control group (P < 0.0001), whereas blood lymphocyte ADA levels as well as blood lymphocyte/serum ADA ratio in the pulmonary TB group were significantly decreased as compared control group (P < 0.0001). In regard to the cutoff value of <34.06 IU/mg protein in patients with pulmonary TB, sensitivity and specificity were 90.00% and 80.00%, respectively.</p><p><strong>Conclusions: </strong>Blood lymphocyte ADA level may be used as a biomarker for the diagnosis of pulmonary TB.</p>\",\"PeriodicalId\":7938,\"journal\":{\"name\":\"Annals of African Medicine\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2025-04-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Annals of African Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4103/aam.aam_212_24\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MEDICINE, GENERAL & INTERNAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annals of African Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/aam.aam_212_24","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}
Diagnostic Accuracy of Adenosine Deaminase Levels in Peripheral Blood Lymphocyte of Patients with Pulmonary Tuberculosis.
Context: Tuberculosis (TB) is a well-known global health problem with one-fifth of the global burden in India. Although there are diagnostic and prognostic markers for TB, there is lack of specificity and sensitivity of diagnostic methods.
Aims: The goal of this study was to assess the diagnostic value of blood lymphocyte adenosine deaminase (ADA) levels in pulmonary TB patients.
Subjects and methods: This was a prospective observational case-control study. A total of 80 subjects were included in this study and were divided into two groups: 40 people who were healthy in every respect and 40 patients with pulmonary TB. ADA activity was measured in each group by the Giusti and Galanti method using a commercial kit.
Statistical analysis used: Data were analyzed using t-test with receiver operating characteristic curve analysis done to identify the cutoff values for ADA that had maximum sensitivity and specificity.
Results: The mean serum ADA level in the pulmonary TB group was significantly increased as compared to control group (P < 0.0001), whereas blood lymphocyte ADA levels as well as blood lymphocyte/serum ADA ratio in the pulmonary TB group were significantly decreased as compared control group (P < 0.0001). In regard to the cutoff value of <34.06 IU/mg protein in patients with pulmonary TB, sensitivity and specificity were 90.00% and 80.00%, respectively.
Conclusions: Blood lymphocyte ADA level may be used as a biomarker for the diagnosis of pulmonary TB.
期刊介绍：
The Annals of African Medicine is published by the Usmanu Danfodiyo University Teaching Hospital, Sokoto, Nigeria and the Annals of African Medicine Society. The Journal is intended to serve as a medium for the publication of research findings in the broad field of Medicine in Africa and other developing countries, and elsewhere which have relevance to Africa. It will serve as a source of information on the state of the art of Medicine in Africa, for continuing education for doctors in Africa and other developing countries, and also for the publication of meetings and conferences. The journal will publish articles I any field of Medicine and other fields which have relevance or implications for Medicine.
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