鲤鱼鱼片氨基酸组成的基因组和转录组学分析。

IF 2.7 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Animals Pub Date : 2025-05-06 DOI:10.3390/ani15091335
Yingjie Chen, Kaikuo Wang, Qi Wang, Yiming Cao, Ran Zhao, Yan Zhang, Jiongtang Li
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引用次数: 0

摘要

鱼类含有丰富的氨基酸,尤其是人体必需氨基酸(HEAAs)。探索鱼片中AA组合含量变化的调控机制,提高养殖鱼片中AA尤其是heaa的含量,对满足人类营养需求具有重要意义。对304条鲤鱼鱼片进行热酸水解后,定量测定了17个单AA和5个AA组的含量,发现它们之间存在显著差异。除Pro外,其余16个AA组和所有AA组的遗传力均在0.2以上。通过全基因组关联研究(GWAS),我们确定了1974个snp和候选基因与至少一种AA含量相关。利用每种AA含量最高和最低组的转录组数据,7089个候选基因与至少两种AA的浓度相关。就HEAA总含量而言,121个snp及其相关基因偏好atp酶偶联跨膜转运蛋白活性,4727个差异表达基因在细胞因子活性、趋化因子活性、氧化还原酶活性和离子结合方面富集。在优化的基因组选择程序和相关snp下,实际AA含量与育种估价值呈高正相关(0.76 ~ 0.90)。这些结果揭示了鱼片AA含量差异的主要影响过程和调控机制。该基因组选择程序将指导未来高AA含量鲤鱼的选择。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genomic and Transcriptomic Profiling of Amino Acid Compositions in Common Carp Fillets.

Fish are rich sources of amino acids (AAs), particularly human essential amino acids (HEAAs). Exploring the regulatory mechanisms behind the changes in the combined AA content in the fillet and enhancing the content of AAs, especially HEAAs, in fillets of farmed fish is crucial for meeting human nutritional needs. After hot acidic hydrolysis of 304 common carp fillets, we quantified the contents of 17 single AAs and 5 AA groups and observed significant variations among them. Except for Pro, 16 single AAs and all AA groups showed medium-to-high heritabilities over 0.2. Through a genome-wide association study (GWAS), we identified 1974 SNPs and candidate genes associated with at least one AA content. Using transcriptome data from groups with the highest and lowest contents for each AA, 7089 candidate genes were related to the concentrations of at least two AAs. For the total HEAA content, 121 SNPs and their associated genes preferred ATPase-coupled transmembrane transporter activity, and 4727 differentially expressed genes were enriched in cytokine activity, chemokine activity, oxidoreductase activity, and ion binding. With the optimal genomic selection programs and associated SNPs, the correlation between the actual AA contents and estimated breeding values was high and positive, ranging from 0.76 to 0.90. These findings revealed the major-effect processes and regulatory mechanisms modulating the differences in fillet AA contents. The genomic selection programs will guide the future selection of common carp with high AA contents.

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来源期刊
Animals
Animals Agricultural and Biological Sciences-Animal Science and Zoology
CiteScore
4.90
自引率
16.70%
发文量
3015
审稿时长
20.52 days
期刊介绍: Animals (ISSN 2076-2615) is an international and interdisciplinary scholarly open access journal. It publishes original research articles, reviews, communications, and short notes that are relevant to any field of study that involves animals, including zoology, ethnozoology, animal science, animal ethics and animal welfare. However, preference will be given to those articles that provide an understanding of animals within a larger context (i.e., the animals'' interactions with the outside world, including humans). There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental details and/or method of study, must be provided for research articles. Articles submitted that involve subjecting animals to unnecessary pain or suffering will not be accepted, and all articles must be submitted with the necessary ethical approval (please refer to the Ethical Guidelines for more information).
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