Reprogramming of Metabolome and Transcriptome Shaped the Elevational Adaptation of Quercus variabilis by Regulating Leaf Functional Traits

Exploring how plants adapt to environmental changes is key to plant survive and protection under accelerating climate change. Quercus variabilis is widely distributed in China with high economic and ecological value, yet its elevational adaptation mechanism remains unclear. Here, we investigated the leaf functional traits, metabolome and transcriptome of Q. variabilis along an elevational gradient (800–1400 m) in Mt. Li, China. Results showed that leaves at higher elevations became smaller, narrower, thicker, with smaller and denser stomata, and maintained higher levels of nitrogen, soluble sugar, total phenol, lignin and soluble sugar-to-starch ratio. With increasing elevation, Q. variabilis underwent a metabolic shift from being dominated by primary metabolism to secondary metabolism, and 1300 m could be identified as the transition point. Particularly, phenylpropanoid metabolism and its metabolites (flavonoids and phenolic acids) played crucial roles in its adaptation to elevations. Moreover, 24 hub transcription factors (TFs) were screened through WGCNA and verified by RT-qPCR. Environmental factors not only directly influenced leaf functional traits, but also affected metabolite accumulation through TF-mediated gene expression, which in turn influenced leaf functional traits. This study highlights that integrating plant functional traits, metabolome and transcriptome simultaneously provides novel insights into the mechanisms for shaping plants’ adaptability.