ELISA和组织病理学诊断猫感染性腹膜炎的敏感性和特异性比较。

Q3 Veterinary
Archives of Razi Institute Pub Date : 2024-10-31 eCollection Date: 2024-10-01 DOI:10.32592/ARI.2024.79.5.1047
S Saeedi, S Safi, S Mashhadirafiee, F Kabir, S P Mortazavi
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引用次数: 0

摘要

猫传染性腹膜炎(FIP)是猫中最常见的病毒性传染病之一。它在诊断方面为兽医提出了许多挑战。本研究的目的是比较ELISA法与组织病理学法的敏感性和特异性。2013年1月至2015年1月,在伊朗德黑兰西北动物诊所,从28只猫身上获得了与猫传染性腹膜炎(FIP)症状一致的样本。在接受检查的猫中,5只被认为是健康的,14只表现出湿性FIP的迹象,9只表现出干性FIP的症状。此外,还确定了生化参数的敏感性和特异性。ELISA检测诊断渗出性FIP的敏感性和特异性均为100%,与组织病理学结果一致。AST (AUC=0.708)和总胆红素(AUC=0.74)诊断FIP的临床准确性中等。阳性猫和阴性对照组的光密度(ODs)在渗出型和非渗出型FIP之间没有统计学差异。采用约登指数(Youden index)确定阳性和阴性猫ODs比值的最佳截断点,估计为3.375。综上所述,ELISA对渗出性FIP的诊断具有较高的预测价值,具有应用于猫冠状病毒感染的血清学诊断的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparison of Sensitivities and Specificities of ELISA and Histopathology to Diagnose Feline Infectious Peritonitis.

Feline infectious peritonitis (FIP) is one of the most prevalent viral infectious diseases in cats. It presents a number of challenges for veterinarians in terms of diagnosis. The objective of this study was to compare the sensitivity and specificity of ELISA with that of histopathology. Samples were obtained from 28 cats exhibiting signs consistent with feline infectious peritonitis (FIP) at the northwest animal clinics in Tehran, Iran, between January 2013 and 2015. Of the cats examined, five were deemed healthy, 14 exhibited indications of wet FIP, and nine displayed symptoms of dry FIP. Furthermore, the sensitivities and specificities of biochemical parameters were determined. The sensitivity and specificity of the ELISA test for diagnosing effusive FIP were found to be 100%, which was identical to the results obtained from histopathology. The AST (AUC=0.708) and total bilirubin (AUC=0.74) demonstrated moderate clinical accuracy in diagnosing FIP. The optical densities (ODs) in positive cats and the negative control group exhibited no statistically significant difference between the effusive and non-effusive forms of FIP. The Youden index was employed to determine the optimal cut-off point for the ratio of ODs in positive and negative cats, which was estimated to be 3.375. In conclusion, the ELISA demonstrated high predictive values for the diagnosis of effusive FIP and has the potential for use in the serological diagnosis of feline coronavirus infection.

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来源期刊
Archives of Razi Institute
Archives of Razi Institute Veterinary-Veterinary (all)
CiteScore
1.50
自引率
0.00%
发文量
108
审稿时长
12 weeks
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