利用含有α-氨基异丁酸的富含精氨酸的细胞穿透L/ d肽传递质粒DNA。

IF 2.9 3区化学 Q1 CHEMISTRY, ORGANIC

Organic & Biomolecular Chemistry Pub Date : 2025-05-06 DOI:10.1039/D5OB00627A

Tomohiro Umeno, Hiroyasu Takemoto and Makoto Oba

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引用次数: 0

摘要

研究了含α、α-二取代α-氨基酸的富含精氨酸的细胞穿透L/ d肽在质粒DNA （pDNA）递送中的折叠行为与细胞内摄取效率的关系。由pDNA和L/ d肽形成的纳米级复合物无论肽的构象如何都能有效地穿过细胞膜。这一发现与先前报道的使用L-和d -氨基酸的细胞穿透肽的共价货物递送方法有很大的不同。

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Plasmid DNA delivery using arginine-rich cell-penetrating l/d-peptides containing α-aminoisobutyric acids†

The relationship between intracellular uptake efficacy and the folding behavior of arginine-rich cell-penetrating L/D-peptides with α,α-disubstituted α-amino acids in plasmid DNA (pDNA) delivery was examined. Nano-sized complexes formed from pDNA and L/D-peptides efficiently traversed the cell membrane regardless of the peptide conformation. This finding represents a significant deviation from previously reported covalent cargo delivery methods using cell penetrating peptides with L- and D-amino acids.

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来源期刊

Organic & Biomolecular Chemistry 化学-有机化学

CiteScore

5.50

自引率

9.40%

发文量

1056

审稿时长

1.3 months

期刊介绍： Organic & Biomolecular Chemistry is an international journal using integrated research in chemistry-organic chemistry. Founded in 2003 by the Royal Society of Chemistry, the journal is published in Semimonthly issues and has been indexed by SCIE, a leading international database. The journal focuses on the key research and cutting-edge progress in the field of chemistry-organic chemistry, publishes and reports the research results in this field in a timely manner, and is committed to becoming a window and platform for rapid academic exchanges among peers in this field. The journal's impact factor in 2023 is 2.9, and its CiteScore is 5.5.