蒙古‘草根’提取物对减少瘤胃甲烷排放及微生物群落的影响

IF 2.5 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Animal Bioscience Pub Date : 2025-10-01 Epub Date: 2025-04-28 DOI:10.5713/ab.24.0836
Xinrui Zhao, Otgonpurev Sukhbaatar, Linlin Kou, Xinming Cheng, Metha Wanapat, Mahmoud Kamal, Togtokhbayar Norovsambuu, Zhanying Sun, Yanfen Cheng
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引用次数: 0

摘要

目的:研究蒙古植物对放牧系统甲烷排放、发酵参数和微生物群落的影响。方法:首先,筛选各种蒙古植物,以确定那些有效地减少体外甲烷产量。选择最有效的植物提取其活性成分,得到具有定量生物活性化合物浓度的固体提取物。分别添加0、1.0、2.0、3.0、4.0和5.0% DM提取物,以稻草和苜蓿为底物,进行体外瘤胃发酵试验,评价其甲烷还原效果。评估的关键指标包括产气量、甲烷产量和瘤胃发酵参数。发酵实验结束后,我们从瘤胃液中提取DNA进行定量PCR和16S高通量测序分析。结果:根(Bergenia crassifolia L.)被鉴定为具有最显著的甲烷还原能力的植物,其提取物中浓缩单宁含量约为70%。结论:与优质粗饲料相比,粗草根对低品质粗饲料甲烷排放的抑制作用更显著,低品质饲料的最佳添加浓度为1.0%。这种植物为减少放牧系统中瘤胃甲烷排放提供了一种有希望的新策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of Mongolian Bergenia crassifolia L. (root) extract on rumen methane emission and microbial community.

Objective: The study aims to research the effects of Mongolian plants on reducing methane emissions, fermentation parameters, and microbial communities in grazing systems.

Methods: Initially, various Mongolian plants were screened to assess their ability to reduce methane production in an in vitro experiment. The most effective plant was selected for extracting active components, and their concentrations were determined. In vitro rumen fermentation used rice straw and alfalfa as substrates. Extract were added at 0%, 1%, 2%, 3%, 4%, and 5% of dry matter. Measured parameters included gas production, methane production, and rumen fermentation parameters. After the fermentation experiments, we extracted DNA from the rumen fluid for quantitative PCR and 16S high-throughput sequencing analysis.

Results: Bergenia crassifolia L. exhibited the most significant methane reduction, its root extract containing approximately 70% condensed tannin, decreased methane production in the rice straw substrate (p<0.01). The 5% addition also showed notable effcacy in the alfalfa substrate (p<0.01). Furthermore, 1% addition of the extract had no significant effect on rumen fermentation parameters. Ruminococcus and Christensenellaceae R-7 were emerged as key methane-reducing taxa (p<0.01).

Conclusion: Bergenia crassifolia L. (root) extract demonstrated stronger methane reduction efficacy in low-quality roughage compared to high-quality roughage, with 1% being the optimal dose. This finding suggests Bergenia crassifolia L. potential as a new strategy for sustainable rumen methane mitigation in grazing systems.

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来源期刊
Animal Bioscience
Animal Bioscience AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
5.00
自引率
0.00%
发文量
223
审稿时长
3 months
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