质谱法表征脂多糖和脂质A的研究进展。

IF 6.6 2区 化学 Q1 SPECTROSCOPY
Amanda Helms, Jennifer S Brodbelt
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引用次数: 0

摘要

脂多糖(LPS)是包裹在革兰氏阴性菌外膜上的复杂分子。LPS在物种和菌株之间具有高度异质性,这对分析提出了重大挑战。这篇综述文章探讨了最近在鉴定和表征LPS的进展,特别侧重于质谱(MS)技术的作用。这篇综述强调了MS如何结合各种分离方法,跨越不同的电离技术和解离模式,使LPS组成的测定更加精确和敏感,重点是脂质a结构。最后,讨论了质谱应用于LPS研究的新趋势,并讨论了其为病原菌抗生素耐药性的发展提供更深入见解的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Progress in Characterization of Lipopolysaccharides and Lipid A by Mass Spectrometry.

Lipopolysaccharides (LPS) are complex molecules embedded in the outer membrane of Gram-negative bacteria. LPS are highly heterogeneous across species and strains, posing a significant analytical challenge. This review article explores recent advances in the identification and characterization of LPS, with a particular focus on the role of mass spectrometry (MS) techniques. The review highlights how MS, in conjunction with various separation methods and spanning different ionization techniques and dissociation modes, has enabled more precise and sensitive determination of LPS composition, with a focus on lipid A structure. Finally, emerging trends in MS applications for LPS research are discussed and its potential to provide deeper insights into the development of antibiotic resistance among pathogenic bacteria.

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来源期刊
Mass Spectrometry Reviews
Mass Spectrometry Reviews 物理-光谱学
CiteScore
16.30
自引率
3.00%
发文量
56
期刊介绍: The aim of the journal Mass Spectrometry Reviews is to publish well-written reviews in selected topics in the various sub-fields of mass spectrometry as a means to summarize the research that has been performed in that area, to focus attention of other researchers, to critically review the published material, and to stimulate further research in that area. The scope of the published reviews include, but are not limited to topics, such as theoretical treatments, instrumental design, ionization methods, analyzers, detectors, application to the qualitative and quantitative analysis of various compounds or elements, basic ion chemistry and structure studies, ion energetic studies, and studies on biomolecules, polymers, etc.
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