高效液相色谱-光电二极管阵列检测器(HPLC-DAD)析因优化及低温分离Pequi纸浆农药残留的验证

IF 4.3 3区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY
ACS Omega Pub Date : 2025-04-22 eCollection Date: 2025-05-06 DOI:10.1021/acsomega.5c01790
Marcelo O Ferreira, João Pedro M Silva, Mara R R Pereira, Allyson L R Santos, Anizio M Faria
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引用次数: 0

摘要

在本研究中,我们建立了低温分配固液萃取法,从枇杷纸浆中提取6种农药残留(多菌灵、二恶龙、carboxin、毒死蜱、terbutylazine和thiabendazole)。Pequi是巴西塞拉多生物群落的主要水果,其特点是农业活动密集。选择这种样品制备技术是因为它简单,分析人员干预最少,并且能有效地从食品样品中提取农药残留。所选择的农药反映了塞拉多生物群系内谷物和甘蔗单一栽培中广泛使用的农药。采用实验设计法对枇杷果肉样品中农药提取工艺进行了优化。优化条件为:样品500 mg、乙酸乙酯7.2 mL、水3.4 mL、冷冻时间3 h,农药回收率为86 ~ 118%。验证的方法无需额外的清理操作,只需一步即可完成,具有选择性、稳健性,定量限低于6.9 μg kg-1,效率高。在塞拉多的战略城市收集的pequi样本中未检测到农药残留。这种缺失可能归因于厚的外外果皮的保护性,它可以保护果肉免受农药污染,即使在农业活动密集的地区也是如此。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Factorial Optimization and Validation of Solid-Liquid Extraction with Low-Temperature Partitioning of Pesticide Residues from Pequi Pulp Using High-Performance Liquid Chromatography with a Photodiode Array Detector (HPLC-DAD).

In this study, we developed a solid-liquid extraction method with low-temperature partitioning for extracting six pesticide residues (carbendazim, diuron, carboxin, chlorpyrifos, terbutylazine, and thiabendazole) from pequi pulp. Pequi is a key fruit of the Brazilian Cerrado biome, which is characterized by its intensive agricultural activity. This sample preparation technique was chosen for its simplicity, minimal analyst intervention, and effective extraction of pesticide residues from food samples. The selected pesticides reflect those widely used in grain and sugar cane monocultures within the Cerrado biome. The optimization of pesticide extraction from pequi pulp samples was conducted by using experimental design approaches. Pesticide recoveries ranged from 86 to 118% under optimized conditions, which included 500 mg of sample, 7.2 mL of ethyl acetate, 3.4 mL of water, and a freezing time of 3 h. The validated method, performed in a single step without additional cleanup procedures, demonstrated selectivity, robustness, and limits of quantification below 6.9 μg kg-1, as well as high efficiency. No pesticide residues were detected in the pequi samples collected from strategic cities in the Cerrado. This absence may be attributed to the protective nature of the thick external epicarp, which shields the pulp from pesticide contamination, even in areas with intensive agricultural activity.

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来源期刊
ACS Omega
ACS Omega Chemical Engineering-General Chemical Engineering
CiteScore
6.60
自引率
4.90%
发文量
3945
审稿时长
2.4 months
期刊介绍: ACS Omega is an open-access global publication for scientific articles that describe new findings in chemistry and interfacing areas of science, without any perceived evaluation of immediate impact.
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