生物容许和亲水的G-CNNPs与抗癌药物依托泊苷的非合作结合和光动力治疗。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
ACS Applied Bio Materials Pub Date : 2025-05-19 Epub Date: 2025-04-11 DOI:10.1021/acsabm.5c00470
Anusree S Gangadharan, Daniel T Thangadurai, Valarmani M Vasanthakannan, Kittusamy Senthilkumar, Devaraj Nataraj
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引用次数: 0

摘要

依托泊苷(ETO)是一种用于肺癌的化疗药物,需要精确和及时的检测来优化癌症管理和减轻毒性。在这项研究中,我们提出了一种可扩展的固态方法,用于合成高度亲水性(平均接触角10.73°)的石墨氮化碳纳米颗粒(g-CNNPs),采用尿素和柠檬酸三钠。合成的g-CNNPs具有6个表面活性位点,可作为检测肺癌药物ETO生理ph值的有效荧光传感器。g-CNNPs对ETO检测具有高选择性和灵敏度(ΦF 20.29→17.95%),检出限(LoD)为95 pM (R2 = 0.99144),定量限(LoQ)为310 pM,关联常数(Ka)为1.0162 M-1。g-CNNPs被ETO猝灭的原因是分子间氢键的静态猝灭和g-CNNPs·ETO配合物内部的非合作结合机制。此外,时间相关单光子计数(TCSPC)分析证实了g-CNNPs在ETO检测时的静态猝灭(寿命为5.175→5.281 ns)。g-CNNPs·ETO复合物的形成通过DFT研究和一系列物理化学表征技术(包括XRD, FE-SEM, HR-TEM, XPS, Raman, FT-IR和UV-vis)得到验证。结果表明,该方法可有效鉴别尿样品中的ETO,回收率在95.45% ~ 110.78%之间。为了评估它们的生物学功效,我们进行了一系列实验,包括对小鼠成纤维细胞系L929的MTT细胞毒性实验、g-CNNPs对HT29细胞(光照和不光照)的抗癌活性以及ROS的生成。总的来说,这些真实样本和生物学研究的结果证实,生物允许的g-CNNPs是临床试验的有希望的候选者。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Biopermissible and Hydrophilic G-CNNPs for Noncooperative Binding with Picomolar of Cancer Drug Etoposide and Photodynamic Therapy.

Etoposide (ETO), a chemotherapeutic agent for lung cancer, requires precise and prompt detection to optimize cancer management and mitigate toxicity. In this study, we present a scalable solid-state methodology for the synthesis of highly hydrophilic (average contact angle 10.73°) graphitic carbon nitride nanoparticles (g-CNNPs) employing urea and trisodium citrate. The synthesized g-CNNPs possess six surface active sites, enabling their function as effective fluorescence sensors for detecting the lung cancer drug ETO at physiological pH. The g-CNNPs demonstrate high selectivity and sensitivity for ETO detection (ΦF 20.29 → 17.95%), with a detection limit (LoD) of 95 pM (R2 = 0.99144), quantification limit (LoQ) of 310 pM, and an association constant (Ka) of 1.0162 M-1. The fluorescence quenching of g-CNNPs by ETO is attributed to intermolecular hydrogen bonding, characterized by static quenching and a noncooperative binding mechanism within the g-CNNPs·ETO complex. Additionally, time-correlated single photon counting (TCSPC) analysis confirms the static quenching of g-CNNPs (lifetime 5.175 → 5.281 ns) upon ETO detection. The formation of the g-CNNPs·ETO complex is verified through DFT studies and a range of physicochemical characterization techniques, including XRD, FE-SEM, HR-TEM, XPS, Raman, FT-IR, and UV-vis. The developed detection method proved effective in identifying ETO in urine samples, achieving high recovery rates between 95.45% and 110.78%. To evaluate their biological efficacy, a series of experiments were conducted, including MTT cytotoxicity assays against mouse fibroblast cell lines L929, the anticancer activity of g-CNNPs toward HT29 cells (with and without light exposure), and ROS generation. Collectively, the results from these real samples and biological studies affirm that biopermissible g-CNNPs are promising candidates for clinical trials.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
期刊介绍: ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.
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