Leonardo Boccoli, Elena Drago, Andrea Cafarelli, Lorenzo Vannozzi, Angelo Sciullo, Federica Iberite, Sajedeh Kerdegari, Toshinori Fujie, Emanuele Gruppioni, Claudio Canale, Leonardo Ricotti
{"title":"掺杂钛酸钡纳米颗粒的微图型苯乙烯-丁二烯-苯乙烯薄膜:对成肌细胞分化的影响。","authors":"Leonardo Boccoli, Elena Drago, Andrea Cafarelli, Lorenzo Vannozzi, Angelo Sciullo, Federica Iberite, Sajedeh Kerdegari, Toshinori Fujie, Emanuele Gruppioni, Claudio Canale, Leonardo Ricotti","doi":"10.1021/acsbiomaterials.4c02468","DOIUrl":null,"url":null,"abstract":"<p><p>Biohybrid actuators exploit the contraction of biological components (muscle cells) to produce a force. In particular, bottom-up approaches use tissue engineering techniques, by coupling cells with a proper scaffold to obtain constructs undergoing contraction and guaranteeing actuation in biohybrid devices. However, the fabrication of actuators able to recapitulate the organization and maturity of native muscle is not trivial. In this field, quasi-two-dimensional (2D) substrates are raising interest due to their high surface/thickness ratio and the possibility of functionalizing their surface. In this work, we fabricated micropatterned thin films made of poly(styrene-butadiene-styrene) (SBS) doped with barium titanate nanoparticles (BTNPs) for fostering myogenic differentiation. We investigated material concentrations and fabrication process parameters to obtain thin microgrooved films with an average thickness below 1 μm, thus featured by a relatively low flexural rigidity and with an anisotropic topography to guide cell alignment and myotube formation. The embodiment of BTNPs did not significantly affect the film's mechanical properties. Interestingly, the presence of BTNPs enhanced the expression of myogenic differentiation markers (i.e., MYH1, MYH4, MYH8, and ACTA1). The results show the promising potential of SBS thin films doped with BTNPs, opening avenues in the fields of biohybrid actuation and skeletal muscle tissue engineering.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 5","pages":"2910-2921"},"PeriodicalIF":5.4000,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12076278/pdf/","citationCount":"0","resultStr":"{\"title\":\"Micropatterned Styrene-Butadiene-Styrene Thin Films Doped with Barium Titanate Nanoparticles: Effects on Myoblast Differentiation.\",\"authors\":\"Leonardo Boccoli, Elena Drago, Andrea Cafarelli, Lorenzo Vannozzi, Angelo Sciullo, Federica Iberite, Sajedeh Kerdegari, Toshinori Fujie, Emanuele Gruppioni, Claudio Canale, Leonardo Ricotti\",\"doi\":\"10.1021/acsbiomaterials.4c02468\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Biohybrid actuators exploit the contraction of biological components (muscle cells) to produce a force. In particular, bottom-up approaches use tissue engineering techniques, by coupling cells with a proper scaffold to obtain constructs undergoing contraction and guaranteeing actuation in biohybrid devices. However, the fabrication of actuators able to recapitulate the organization and maturity of native muscle is not trivial. In this field, quasi-two-dimensional (2D) substrates are raising interest due to their high surface/thickness ratio and the possibility of functionalizing their surface. In this work, we fabricated micropatterned thin films made of poly(styrene-butadiene-styrene) (SBS) doped with barium titanate nanoparticles (BTNPs) for fostering myogenic differentiation. We investigated material concentrations and fabrication process parameters to obtain thin microgrooved films with an average thickness below 1 μm, thus featured by a relatively low flexural rigidity and with an anisotropic topography to guide cell alignment and myotube formation. The embodiment of BTNPs did not significantly affect the film's mechanical properties. Interestingly, the presence of BTNPs enhanced the expression of myogenic differentiation markers (i.e., MYH1, MYH4, MYH8, and ACTA1). The results show the promising potential of SBS thin films doped with BTNPs, opening avenues in the fields of biohybrid actuation and skeletal muscle tissue engineering.</p>\",\"PeriodicalId\":8,\"journal\":{\"name\":\"ACS Biomaterials Science & Engineering\",\"volume\":\"11 5\",\"pages\":\"2910-2921\"},\"PeriodicalIF\":5.4000,\"publicationDate\":\"2025-05-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12076278/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Biomaterials Science & Engineering\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1021/acsbiomaterials.4c02468\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/5/1 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Biomaterials Science & Engineering","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1021/acsbiomaterials.4c02468","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/5/1 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
Micropatterned Styrene-Butadiene-Styrene Thin Films Doped with Barium Titanate Nanoparticles: Effects on Myoblast Differentiation.
Biohybrid actuators exploit the contraction of biological components (muscle cells) to produce a force. In particular, bottom-up approaches use tissue engineering techniques, by coupling cells with a proper scaffold to obtain constructs undergoing contraction and guaranteeing actuation in biohybrid devices. However, the fabrication of actuators able to recapitulate the organization and maturity of native muscle is not trivial. In this field, quasi-two-dimensional (2D) substrates are raising interest due to their high surface/thickness ratio and the possibility of functionalizing their surface. In this work, we fabricated micropatterned thin films made of poly(styrene-butadiene-styrene) (SBS) doped with barium titanate nanoparticles (BTNPs) for fostering myogenic differentiation. We investigated material concentrations and fabrication process parameters to obtain thin microgrooved films with an average thickness below 1 μm, thus featured by a relatively low flexural rigidity and with an anisotropic topography to guide cell alignment and myotube formation. The embodiment of BTNPs did not significantly affect the film's mechanical properties. Interestingly, the presence of BTNPs enhanced the expression of myogenic differentiation markers (i.e., MYH1, MYH4, MYH8, and ACTA1). The results show the promising potential of SBS thin films doped with BTNPs, opening avenues in the fields of biohybrid actuation and skeletal muscle tissue engineering.
期刊介绍:
ACS Biomaterials Science & Engineering is the leading journal in the field of biomaterials, serving as an international forum for publishing cutting-edge research and innovative ideas on a broad range of topics:
Applications and Health – implantable tissues and devices, prosthesis, health risks, toxicology
Bio-interactions and Bio-compatibility – material-biology interactions, chemical/morphological/structural communication, mechanobiology, signaling and biological responses, immuno-engineering, calcification, coatings, corrosion and degradation of biomaterials and devices, biophysical regulation of cell functions
Characterization, Synthesis, and Modification – new biomaterials, bioinspired and biomimetic approaches to biomaterials, exploiting structural hierarchy and architectural control, combinatorial strategies for biomaterials discovery, genetic biomaterials design, synthetic biology, new composite systems, bionics, polymer synthesis
Controlled Release and Delivery Systems – biomaterial-based drug and gene delivery, bio-responsive delivery of regulatory molecules, pharmaceutical engineering
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Manufacturing and Technology – 3D printing, inks, organ-on-a-chip, bioreactor/perfusion systems, microdevices, BioMEMS, optics and electronics interfaces with biomaterials, systems integration
Modeling and Informatics Tools – scaling methods to guide biomaterial design, predictive algorithms for structure-function, biomechanics, integrating bioinformatics with biomaterials discovery, metabolomics in the context of biomaterials
Tissue Engineering and Regenerative Medicine – basic and applied studies, cell therapies, scaffolds, vascularization, bioartificial organs, transplantation and functionality, cellular agriculture