掺杂钛酸钡纳米颗粒的微图型苯乙烯-丁二烯-苯乙烯薄膜:对成肌细胞分化的影响。

IF 5.4 2区 医学 Q2 MATERIALS SCIENCE, BIOMATERIALS
Leonardo Boccoli, Elena Drago, Andrea Cafarelli, Lorenzo Vannozzi, Angelo Sciullo, Federica Iberite, Sajedeh Kerdegari, Toshinori Fujie, Emanuele Gruppioni, Claudio Canale, Leonardo Ricotti
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引用次数: 0

摘要

生物混合驱动器利用生物成分(肌肉细胞)的收缩来产生力。特别是,自下而上的方法使用组织工程技术,通过将细胞与适当的支架偶联来获得在生物混合装置中进行收缩并保证驱动的结构。然而,制造能够再现天然肌肉组织和成熟的致动器并非易事。在这一领域,准二维(2D)衬底由于其高表面/厚度比和表面功能化的可能性而引起了人们的兴趣。在这项工作中,我们制备了掺杂钛酸钡纳米颗粒(BTNPs)的聚(苯乙烯-丁二烯-苯乙烯)(SBS)制成的微图案薄膜,以促进肌分化。我们研究了材料浓度和制备工艺参数,获得了平均厚度小于1 μm的微沟槽薄膜,该薄膜具有相对较低的抗弯刚度和各向异性的形貌,可引导细胞排列和肌管形成。BTNPs的加入对薄膜的力学性能没有显著影响。有趣的是,BTNPs的存在增强了肌源性分化标志物(即MYH1、MYH4、MYH8和ACTA1)的表达。结果表明,掺BTNPs的SBS薄膜在生物混合驱动和骨骼肌组织工程领域具有广阔的应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Micropatterned Styrene-Butadiene-Styrene Thin Films Doped with Barium Titanate Nanoparticles: Effects on Myoblast Differentiation.

Biohybrid actuators exploit the contraction of biological components (muscle cells) to produce a force. In particular, bottom-up approaches use tissue engineering techniques, by coupling cells with a proper scaffold to obtain constructs undergoing contraction and guaranteeing actuation in biohybrid devices. However, the fabrication of actuators able to recapitulate the organization and maturity of native muscle is not trivial. In this field, quasi-two-dimensional (2D) substrates are raising interest due to their high surface/thickness ratio and the possibility of functionalizing their surface. In this work, we fabricated micropatterned thin films made of poly(styrene-butadiene-styrene) (SBS) doped with barium titanate nanoparticles (BTNPs) for fostering myogenic differentiation. We investigated material concentrations and fabrication process parameters to obtain thin microgrooved films with an average thickness below 1 μm, thus featured by a relatively low flexural rigidity and with an anisotropic topography to guide cell alignment and myotube formation. The embodiment of BTNPs did not significantly affect the film's mechanical properties. Interestingly, the presence of BTNPs enhanced the expression of myogenic differentiation markers (i.e., MYH1, MYH4, MYH8, and ACTA1). The results show the promising potential of SBS thin films doped with BTNPs, opening avenues in the fields of biohybrid actuation and skeletal muscle tissue engineering.

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来源期刊
ACS Biomaterials Science & Engineering
ACS Biomaterials Science & Engineering Materials Science-Biomaterials
CiteScore
10.30
自引率
3.40%
发文量
413
期刊介绍: ACS Biomaterials Science & Engineering is the leading journal in the field of biomaterials, serving as an international forum for publishing cutting-edge research and innovative ideas on a broad range of topics: Applications and Health – implantable tissues and devices, prosthesis, health risks, toxicology Bio-interactions and Bio-compatibility – material-biology interactions, chemical/morphological/structural communication, mechanobiology, signaling and biological responses, immuno-engineering, calcification, coatings, corrosion and degradation of biomaterials and devices, biophysical regulation of cell functions Characterization, Synthesis, and Modification – new biomaterials, bioinspired and biomimetic approaches to biomaterials, exploiting structural hierarchy and architectural control, combinatorial strategies for biomaterials discovery, genetic biomaterials design, synthetic biology, new composite systems, bionics, polymer synthesis Controlled Release and Delivery Systems – biomaterial-based drug and gene delivery, bio-responsive delivery of regulatory molecules, pharmaceutical engineering Healthcare Advances – clinical translation, regulatory issues, patient safety, emerging trends Imaging and Diagnostics – imaging agents and probes, theranostics, biosensors, monitoring Manufacturing and Technology – 3D printing, inks, organ-on-a-chip, bioreactor/perfusion systems, microdevices, BioMEMS, optics and electronics interfaces with biomaterials, systems integration Modeling and Informatics Tools – scaling methods to guide biomaterial design, predictive algorithms for structure-function, biomechanics, integrating bioinformatics with biomaterials discovery, metabolomics in the context of biomaterials Tissue Engineering and Regenerative Medicine – basic and applied studies, cell therapies, scaffolds, vascularization, bioartificial organs, transplantation and functionality, cellular agriculture
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