创新膜方法制备辣根过氧化物酶偶联物用于病毒感染检测

IF 1 4区 化学 Q4 CHEMISTRY, ANALYTICAL
V. M. Shkinev, V. F. Larichev, D. A. Trofimov
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引用次数: 0

摘要

提出了一种方法,使用膜过滤的发展方法,以获得辣根过氧化物酶为基础的偶联物检测病毒感染。这种方法明显比色谱法更简单、更快、更具成本效益。在纯化过程中几乎不发生共轭损失。提出了一种膜纯化辣根过氧化物酶及其毛细管电泳分析的方法。该程序还确保制备与病毒抗原结合的辣根过氧化物酶偶联物,用于酶联免疫吸附试验(ELISA),用于检测针对病毒的M类抗体(在M抗体捕获修饰中)。本文描述了利用这种方法制备的各种诊断试剂盒的实践,例如在ELISA测试系统中,用于诊断由西尼罗河病毒、克里米亚-刚果出血热、蜱传脑炎等病毒引起的疾病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Innovative Membrane Approaches to Creating Horseradish Peroxidase-Based Conjugates for the Detection of Viral Infections

An approach is proposed to using membrane filtration in the development of methodologies for obtaining horseradish peroxidase-based conjugates for the detection of viral infections. This approach is significantly simpler, faster, and more cost-effective than chromatographic methods. Nearly no conjugate losses occur during the purification of the components. A procedure for the membrane purification of horseradish peroxidase and its analysis via capillary electrophoresis is proposed. The procedure also ensures the preparation of horseradish peroxidase conjugates with viral antigens for use in enzyme-linked immunosorbent assays (ELISA) for the detection of class M antibodies against a virus (in the M antibody capture modification). The practice of utilizing various diagnostic kits prepared with this approach is described, for example, in ELISA test systems, for diagnosing diseases caused by viruses like West Nile, Crimean–Congo hemorrhagic fever, tick-borne encephalitis, and others.

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来源期刊
Journal of Analytical Chemistry
Journal of Analytical Chemistry 化学-分析化学
CiteScore
2.10
自引率
9.10%
发文量
146
审稿时长
13 months
期刊介绍: The Journal of Analytical Chemistry is an international peer reviewed journal that covers theoretical and applied aspects of analytical chemistry; it informs the reader about new achievements in analytical methods, instruments and reagents. Ample space is devoted to problems arising in the analysis of vital media such as water and air. Consideration is given to the detection and determination of metal ions, anions, and various organic substances. The journal welcomes manuscripts from all countries in the English or Russian language.
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