含有Keap1单体的模块化纳米转运体能够降低对乙酰氨基酚对小鼠肝脏的毒性作用

IF 0.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yu. V. Khramtsov, A. A. Rosenkranz, A. V. Ulasov, T. A. Slastnikova, T. N. Lupanova, R. T. Alieva,  G. P. Georgiev,  A. S. Sobolev
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引用次数: 0

摘要

在此之前,我们创建了一种模块化纳米转运体(MNT),其中包含Keap1的单体,Keap1是Nrf2转录因子的细胞内蛋白抑制剂,控制细胞对氧化应激的保护,并能够与肝细胞中的Keap1相互作用,保护这些细胞免受过氧化氢的影响。以对乙酰氨基酚氧化性肝损伤为模型,研究其抗氧化作用。小鼠腹腔注射对乙酰氨基酚后,血液中丙氨酸转氨酶和天冬氨酸转氨酶水平升高,肝脏水肿加重。在对乙酰氨基酚注射前2小时初步静脉注射MNT后,观察到血液中这些酶的水平显著降低,同时肝脏水肿减少。所获得的结果可作为开发治疗与氧化应激相关疾病的药物的基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Modular Nanotransporters Containing Keap1 Monobodies Are Capable of Reducing the Toxic Effect of Acetaminophen on the Liver of Mice

Previously, we created a modular nanotransporter (MNT) containing a monobody to Keap1, an intracellular protein inhibitor of the Nrf2 transcription factor that controls cellular protection from oxidative stress and is capable of interacting with Keap1 in hepatocytes and protect these cells from the effects of hydrogen peroxide. Oxidative liver damage by acetaminophen was used as a model to study the antitoxic effect of this MNT. Intraperitoneal injection of acetaminophen to mice resulted in an increase in the level of alanine aminotransferase and aspartate aminotransferase in the blood, as well as in liver edema. A significant decrease in the level of these enzymes in the blood, along with a decrease in liver edema, was observed after preliminary intravenous administration of MNT 2 h before the acetaminophen injection. The results obtained can be used as a basis for developing drugs to treat diseases associated with oxidative stress.

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来源期刊
Doklady Biochemistry and Biophysics
Doklady Biochemistry and Biophysics 生物-生化与分子生物学
CiteScore
1.60
自引率
12.50%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.
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