长期长期受辐射的Techa河滨村居民t细胞受体v β段多样性分析

IF 0.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
A. I. Kotikova, E. A. Blinova, A. V. Akleyev
{"title":"长期长期受辐射的Techa河滨村居民t细胞受体v β段多样性分析","authors":"A. I. Kotikova,&nbsp;E. A. Blinova,&nbsp;A. V. Akleyev","doi":"10.1134/S1607672925700048","DOIUrl":null,"url":null,"abstract":"<p>Objective. To study the repertoire of the T-cell receptor in persons chronically exposed to radiation in the long-term period. \n<b>Materials and methods.</b> The study involved 48 people, who were divided into two groups: a group of exposed persons (31 individuals with the average accumulated dose to red bone marrow (RBM) of 981 ± 130 mGy) and a comparison group (17 individuals with the average accumulated dose to RBM of 25.3 ± 5.91 mGy). The study groups did not differ significantly in age, gender and ethnicity. The repertoire of Vβ-segments of the T-cell receptor of the peripheral blood T-lymphocytes of exposed persons was analyzed by flow cytometry method. 24 Vβ-segments of the T-cell receptor were studied. Statistical processing of the obtained data was carried out using the Wilcoxon signed-rank test, and a direct description of Vβ-segment repertoire of the T-cell receptor was performed using the Lorenz curve and the Gini-TCR index. \n<b>Results.</b> The study revealed a statistically significant increase in the number of Vβ3 and Vβ5.2 T-cell receptor segments in exposed individuals relative to the comparison group (<i>p</i> = 0.03 and <i>p</i> = 0.003, respectively). It was also shown that the distribution of the Vβ-segments of the T-cell receptor is uneven in both study groups. However, there was no significant difference between the repertoires of the T-cell receptor of the studied groups in the Gini-TCR index (<i>p</i> = 0.14).</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"521 1","pages":"261 - 266"},"PeriodicalIF":0.8000,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Analysis of Vβ-Segment Diversity of T-Cell Receptor in Residents of the Techa Riverside Villages Chronically Exposed to Radiation in the Long-Term Period\",\"authors\":\"A. I. Kotikova,&nbsp;E. A. Blinova,&nbsp;A. V. Akleyev\",\"doi\":\"10.1134/S1607672925700048\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Objective. To study the repertoire of the T-cell receptor in persons chronically exposed to radiation in the long-term period. \\n<b>Materials and methods.</b> The study involved 48 people, who were divided into two groups: a group of exposed persons (31 individuals with the average accumulated dose to red bone marrow (RBM) of 981 ± 130 mGy) and a comparison group (17 individuals with the average accumulated dose to RBM of 25.3 ± 5.91 mGy). The study groups did not differ significantly in age, gender and ethnicity. The repertoire of Vβ-segments of the T-cell receptor of the peripheral blood T-lymphocytes of exposed persons was analyzed by flow cytometry method. 24 Vβ-segments of the T-cell receptor were studied. Statistical processing of the obtained data was carried out using the Wilcoxon signed-rank test, and a direct description of Vβ-segment repertoire of the T-cell receptor was performed using the Lorenz curve and the Gini-TCR index. \\n<b>Results.</b> The study revealed a statistically significant increase in the number of Vβ3 and Vβ5.2 T-cell receptor segments in exposed individuals relative to the comparison group (<i>p</i> = 0.03 and <i>p</i> = 0.003, respectively). It was also shown that the distribution of the Vβ-segments of the T-cell receptor is uneven in both study groups. However, there was no significant difference between the repertoires of the T-cell receptor of the studied groups in the Gini-TCR index (<i>p</i> = 0.14).</p>\",\"PeriodicalId\":529,\"journal\":{\"name\":\"Doklady Biochemistry and Biophysics\",\"volume\":\"521 1\",\"pages\":\"261 - 266\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2025-04-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Doklady Biochemistry and Biophysics\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1134/S1607672925700048\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Doklady Biochemistry and Biophysics","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1134/S1607672925700048","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

目标。目的:研究长期慢性辐射暴露人群的t细胞受体库。材料和方法。该研究涉及48人,将其分为两组:暴露组(31人,红骨髓平均累积剂量为981±130 mGy)和对照组(17人,红骨髓平均累积剂量为25.3±5.91 mGy)。研究小组在年龄、性别和种族方面没有显著差异。用流式细胞术分析暴露者外周血t淋巴细胞v β受体片段库。研究了t细胞受体的24个v β片段。使用Wilcoxon符号秩检验对所得数据进行统计处理,并使用Lorenz曲线和Gini-TCR指数对t细胞受体的v β片段库进行直接描述。结果。研究显示,与对照组相比,暴露个体的Vβ3和Vβ5.2 t细胞受体片段数量有统计学意义的增加(p = 0.03和p = 0.003)。研究还表明,在两个研究组中,t细胞受体的v β片段的分布是不均匀的。然而,在Gini-TCR指数中,研究组的t细胞受体谱间无显著差异(p = 0.14)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Analysis of Vβ-Segment Diversity of T-Cell Receptor in Residents of the Techa Riverside Villages Chronically Exposed to Radiation in the Long-Term Period

Objective. To study the repertoire of the T-cell receptor in persons chronically exposed to radiation in the long-term period. Materials and methods. The study involved 48 people, who were divided into two groups: a group of exposed persons (31 individuals with the average accumulated dose to red bone marrow (RBM) of 981 ± 130 mGy) and a comparison group (17 individuals with the average accumulated dose to RBM of 25.3 ± 5.91 mGy). The study groups did not differ significantly in age, gender and ethnicity. The repertoire of Vβ-segments of the T-cell receptor of the peripheral blood T-lymphocytes of exposed persons was analyzed by flow cytometry method. 24 Vβ-segments of the T-cell receptor were studied. Statistical processing of the obtained data was carried out using the Wilcoxon signed-rank test, and a direct description of Vβ-segment repertoire of the T-cell receptor was performed using the Lorenz curve and the Gini-TCR index. Results. The study revealed a statistically significant increase in the number of Vβ3 and Vβ5.2 T-cell receptor segments in exposed individuals relative to the comparison group (p = 0.03 and p = 0.003, respectively). It was also shown that the distribution of the Vβ-segments of the T-cell receptor is uneven in both study groups. However, there was no significant difference between the repertoires of the T-cell receptor of the studied groups in the Gini-TCR index (p = 0.14).

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Doklady Biochemistry and Biophysics
Doklady Biochemistry and Biophysics 生物-生化与分子生物学
CiteScore
1.60
自引率
12.50%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信