农药在混合物中相互作用的类型:抑制试验结果

IF 0.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
E. N. Esimbekova, D. V. Satir, V. A. Kratasyuk
{"title":"农药在混合物中相互作用的类型:抑制试验结果","authors":"E. N. Esimbekova,&nbsp;D. V. Satir,&nbsp;V. A. Kratasyuk","doi":"10.1134/S1607672924601422","DOIUrl":null,"url":null,"abstract":"<p>Enzymatic inhibitory assay based on the coupled enzyme system NAD(P)·H:FMN oxidoreductase and luciferase (Red + Luc), originally developed for environmental monitoring of soils, water, and air, is proposed as a method for evaluating changes in the properties of active ingredients of pesticide preparations depending on the additional components (formulants), as well as when pesticides are combined in mixtures. Using the commercial pesticide preparations containing glyphosate, it was shown that the degree of inhibition of the coupled enzyme system Red + Luc largely depends on the formulants rather than on the active ingredient in their composition. Moreover, the combined inhibitory effect of the pesticides mixture on the coupled enzyme system Red + Luc was not additive. According to the results of the inhibitory assay, the type of interaction of pesticide preparations in mixtures depends on both the formulants used and the ratio of pesticides in the mixture.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"521 1","pages":"267 - 271"},"PeriodicalIF":0.8000,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Types of Pesticides Interaction in Mixtures: Results of Inhibitory Assay\",\"authors\":\"E. N. Esimbekova,&nbsp;D. V. Satir,&nbsp;V. A. Kratasyuk\",\"doi\":\"10.1134/S1607672924601422\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Enzymatic inhibitory assay based on the coupled enzyme system NAD(P)·H:FMN oxidoreductase and luciferase (Red + Luc), originally developed for environmental monitoring of soils, water, and air, is proposed as a method for evaluating changes in the properties of active ingredients of pesticide preparations depending on the additional components (formulants), as well as when pesticides are combined in mixtures. Using the commercial pesticide preparations containing glyphosate, it was shown that the degree of inhibition of the coupled enzyme system Red + Luc largely depends on the formulants rather than on the active ingredient in their composition. Moreover, the combined inhibitory effect of the pesticides mixture on the coupled enzyme system Red + Luc was not additive. According to the results of the inhibitory assay, the type of interaction of pesticide preparations in mixtures depends on both the formulants used and the ratio of pesticides in the mixture.</p>\",\"PeriodicalId\":529,\"journal\":{\"name\":\"Doklady Biochemistry and Biophysics\",\"volume\":\"521 1\",\"pages\":\"267 - 271\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2025-04-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Doklady Biochemistry and Biophysics\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1134/S1607672924601422\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Doklady Biochemistry and Biophysics","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1134/S1607672924601422","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

基于NAD(P)·H:FMN氧化还原酶和荧光素酶(Red + Luc)偶联酶系统的酶抑制测定,最初是为土壤、水和空气的环境监测而开发的,现在被提议作为一种评估农药制剂中活性成分性质变化的方法,这种变化取决于附加成分(配方),以及当农药混合在一起时。利用含有草甘膦的商业农药制剂,研究表明,Red + Luc偶联酶系统的抑制程度主要取决于制剂而不是其组成中的活性成分。此外,混合农药对酶偶联体系Red + Luc的联合抑制作用不具有加性。根据抑制试验的结果,混合物中农药制剂的相互作用类型取决于所使用的制剂和混合物中农药的比例。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Types of Pesticides Interaction in Mixtures: Results of Inhibitory Assay

Enzymatic inhibitory assay based on the coupled enzyme system NAD(P)·H:FMN oxidoreductase and luciferase (Red + Luc), originally developed for environmental monitoring of soils, water, and air, is proposed as a method for evaluating changes in the properties of active ingredients of pesticide preparations depending on the additional components (formulants), as well as when pesticides are combined in mixtures. Using the commercial pesticide preparations containing glyphosate, it was shown that the degree of inhibition of the coupled enzyme system Red + Luc largely depends on the formulants rather than on the active ingredient in their composition. Moreover, the combined inhibitory effect of the pesticides mixture on the coupled enzyme system Red + Luc was not additive. According to the results of the inhibitory assay, the type of interaction of pesticide preparations in mixtures depends on both the formulants used and the ratio of pesticides in the mixture.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Doklady Biochemistry and Biophysics
Doklady Biochemistry and Biophysics 生物-生化与分子生物学
CiteScore
1.60
自引率
12.50%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信