{"title":"农药在混合物中相互作用的类型:抑制试验结果","authors":"E. N. Esimbekova, D. V. Satir, V. A. Kratasyuk","doi":"10.1134/S1607672924601422","DOIUrl":null,"url":null,"abstract":"<p>Enzymatic inhibitory assay based on the coupled enzyme system NAD(P)·H:FMN oxidoreductase and luciferase (Red + Luc), originally developed for environmental monitoring of soils, water, and air, is proposed as a method for evaluating changes in the properties of active ingredients of pesticide preparations depending on the additional components (formulants), as well as when pesticides are combined in mixtures. Using the commercial pesticide preparations containing glyphosate, it was shown that the degree of inhibition of the coupled enzyme system Red + Luc largely depends on the formulants rather than on the active ingredient in their composition. Moreover, the combined inhibitory effect of the pesticides mixture on the coupled enzyme system Red + Luc was not additive. According to the results of the inhibitory assay, the type of interaction of pesticide preparations in mixtures depends on both the formulants used and the ratio of pesticides in the mixture.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"521 1","pages":"267 - 271"},"PeriodicalIF":0.8000,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Types of Pesticides Interaction in Mixtures: Results of Inhibitory Assay\",\"authors\":\"E. N. Esimbekova, D. V. Satir, V. A. Kratasyuk\",\"doi\":\"10.1134/S1607672924601422\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Enzymatic inhibitory assay based on the coupled enzyme system NAD(P)·H:FMN oxidoreductase and luciferase (Red + Luc), originally developed for environmental monitoring of soils, water, and air, is proposed as a method for evaluating changes in the properties of active ingredients of pesticide preparations depending on the additional components (formulants), as well as when pesticides are combined in mixtures. Using the commercial pesticide preparations containing glyphosate, it was shown that the degree of inhibition of the coupled enzyme system Red + Luc largely depends on the formulants rather than on the active ingredient in their composition. Moreover, the combined inhibitory effect of the pesticides mixture on the coupled enzyme system Red + Luc was not additive. According to the results of the inhibitory assay, the type of interaction of pesticide preparations in mixtures depends on both the formulants used and the ratio of pesticides in the mixture.</p>\",\"PeriodicalId\":529,\"journal\":{\"name\":\"Doklady Biochemistry and Biophysics\",\"volume\":\"521 1\",\"pages\":\"267 - 271\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2025-04-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Doklady Biochemistry and Biophysics\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1134/S1607672924601422\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Doklady Biochemistry and Biophysics","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1134/S1607672924601422","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Types of Pesticides Interaction in Mixtures: Results of Inhibitory Assay
Enzymatic inhibitory assay based on the coupled enzyme system NAD(P)·H:FMN oxidoreductase and luciferase (Red + Luc), originally developed for environmental monitoring of soils, water, and air, is proposed as a method for evaluating changes in the properties of active ingredients of pesticide preparations depending on the additional components (formulants), as well as when pesticides are combined in mixtures. Using the commercial pesticide preparations containing glyphosate, it was shown that the degree of inhibition of the coupled enzyme system Red + Luc largely depends on the formulants rather than on the active ingredient in their composition. Moreover, the combined inhibitory effect of the pesticides mixture on the coupled enzyme system Red + Luc was not additive. According to the results of the inhibitory assay, the type of interaction of pesticide preparations in mixtures depends on both the formulants used and the ratio of pesticides in the mixture.
期刊介绍:
Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.