子宫内慢性辐射长期暴露人群外周血t辅助细胞亚群组成特征

IF 0.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
A. I. Kotikova, V. S. Nikiforov, E. A. Blinova, A. V. Akleyev
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引用次数: 0

摘要

这项工作的目的是研究外周血中不同的t -辅助性亚群的数量以及TBX21、RORC、GATA3、NFKB1、MAPK8和STAT3基因的表达,这些基因负责调节子宫内慢性暴露者的t -辅助性分化。这项研究的对象是156名在子宫内和出生后长期暴露在大剂量特查河中的人的外周血。受照个体红骨髓平均累积吸收剂量为496±51.2 mGy(剂量范围,73.5 ~ 1298 mGy),对照组1为18.7±1.97 mGy(剂量范围,0.78 ~ 57 mGy),对照组2为571±49.1 mGy(剂量范围,86.74 ~ 1240 mGy)。流式细胞术分析t辅助细胞亚群组成。RT-PCR检测TBX21、RORC、GATA3、NFKB1、MAPK8、STAT3基因相对mRNA含量。在子宫和出生后暴露的个体外周血中,t辅助细胞、效应记忆t辅助细胞和中枢记忆t辅助细胞的总数呈剂量依赖性下降,呈趋势水平,而幼稚t辅助细胞的相对数量增加。与仅在出生后暴露的人群相比,在子宫内和出生后暴露的人群中,1型t辅助细胞的相对数量也有所增加。这些变化并不取决于累积辐射剂量。研究基因GATA3、STAT3、TBX21、MAPK8、RORc的mRNA表达变化无统计学意义。研究基因的mRNA表达与被检测个体中辅助t型1、2和17亚群中细胞的相对数量之间没有相关性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Features of the Subpopulation Composition of Peripheral Blood T-Helpers in the Long-Term Period in Persons Exposed to Chronic Radiation In Utero

The objective of this work was to study the number of different T-helper subpopulations in peripheral blood as well as the expression of TBX21, RORC, GATA3, NFKB1, MAPK8, and STAT3 genes, responsible for the regulation of T-helper differentiation in persons chronically exposed in utero. The object of the study was peripheral blood cells obtained from 156 people chronically exposed in utero and postnatally in a wide range of doses on the Techa River. The mean cumulative absorbed dose to the red bone marrow in the examined exposed individuals was 496 ± 51.2 mGy (dose range, 73.5–1298 mGy), in the comparison group 1 it was 18.7 ± 1.97 mGy (dose range, 0.78–57 mGy), and in comparison group 2 (exposed only postnatally) it was 571 ± 49.1 mGy (dose range, 86.74–1240 mGy). The subpopulation composition of T-helper cells was analyzed by flow cytometry. The relative mRNA content of TBX21, RORC, GATA3, NFKB1, MAPK8, and STAT3 genes was assessed by RT–PCR. A dose-dependent decrease in the total number of T-helper cells, effector memory T-helper cells, and central memory T-helper cells at a trend level and an increase in the relative number of naive T-helper cells in the peripheral blood of individuals exposed in utero and postnatally were found. An increase in the relative number of type 1 T-helper cells was also revealed in those exposed in utero and postnatally relative to the group of persons exposed only postnatally. These changes did not depend on the cumulative radiation doses. There were no statistically significant changes in the mRNA expression of the studied genes (GATA3, STAT3, TBX21, MAPK8, and RORc). No correlation between the mRNA expression of the studied genes and the relative number of cells in the subpopulations of T-helper types 1, 2, and 17 in the examined individuals was revealed.

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来源期刊
Doklady Biochemistry and Biophysics
Doklady Biochemistry and Biophysics 生物-生化与分子生物学
CiteScore
1.60
自引率
12.50%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.
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