{"title":"谷氨酰胺转胺酶和百里香油对蚕豆蛋白基活性膜性能的影响","authors":"Melissa Kocatepe , Kubra Ertan , Serpil Sahin , Gulum Sumnu","doi":"10.1016/j.ijbiomac.2025.144178","DOIUrl":null,"url":null,"abstract":"<div><div>In this study, faba bean (<em>Vicia faba L</em>.) protein concentrate-based biodegradable films were developed using the casting method at two pH levels (6 and 8) and with different microbial transglutaminase (MTGase) concentrations (0, 5 U, and 10 U). Films prepared at pH 8 exhibited improved mechanical properties due to the higher solubility of proteins. Therefore, subsequent experiments were conducted with the films prepared at this pH. The films were evaluated for their barrier properties, color, antioxidant properties, thermal stability, and degree of cross-linking. The incorporation of 5 U MTGase led to random cross-link formation within the film matrix, which negatively impacted the mechanical and barrier properties. In contrast, 10 U MTGase enhanced the physical properties of the films and resulted in a higher degree of cross-linking. Thyme oil (<em>Thymus vulgaris</em>) was incorporated into the film-forming solutions to enhance their antioxidant and antimicrobial properties. While 1 % thyme oil enhanced the active properties of the films, it reduced their mechanical strength, barrier performance, and degree of cross-linking. Despite these challenges, incorporating 1 % thyme oil in films crosslinked with 10 U MTGase is recommended for developing active packaging films with high antioxidant capacity and antimicrobial effectiveness.</div></div>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":"312 ","pages":"Article 144178"},"PeriodicalIF":8.5000,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of transglutaminase and thyme oil addition on the properties of faba bean protein-based active films\",\"authors\":\"Melissa Kocatepe , Kubra Ertan , Serpil Sahin , Gulum Sumnu\",\"doi\":\"10.1016/j.ijbiomac.2025.144178\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>In this study, faba bean (<em>Vicia faba L</em>.) protein concentrate-based biodegradable films were developed using the casting method at two pH levels (6 and 8) and with different microbial transglutaminase (MTGase) concentrations (0, 5 U, and 10 U). Films prepared at pH 8 exhibited improved mechanical properties due to the higher solubility of proteins. Therefore, subsequent experiments were conducted with the films prepared at this pH. The films were evaluated for their barrier properties, color, antioxidant properties, thermal stability, and degree of cross-linking. The incorporation of 5 U MTGase led to random cross-link formation within the film matrix, which negatively impacted the mechanical and barrier properties. In contrast, 10 U MTGase enhanced the physical properties of the films and resulted in a higher degree of cross-linking. Thyme oil (<em>Thymus vulgaris</em>) was incorporated into the film-forming solutions to enhance their antioxidant and antimicrobial properties. While 1 % thyme oil enhanced the active properties of the films, it reduced their mechanical strength, barrier performance, and degree of cross-linking. Despite these challenges, incorporating 1 % thyme oil in films crosslinked with 10 U MTGase is recommended for developing active packaging films with high antioxidant capacity and antimicrobial effectiveness.</div></div>\",\"PeriodicalId\":333,\"journal\":{\"name\":\"International Journal of Biological Macromolecules\",\"volume\":\"312 \",\"pages\":\"Article 144178\"},\"PeriodicalIF\":8.5000,\"publicationDate\":\"2025-05-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Biological Macromolecules\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0141813025047300\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Biological Macromolecules","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0141813025047300","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
本研究以蚕豆(Vicia faba L.)蛋白为基础,在两种pH值(6和8)和不同微生物谷氨酰胺转胺酶(mtase)浓度(0、5和10 U)下采用浇铸法制备了可降解膜。由于蛋白质的溶解度较高,在pH 8下制备的膜表现出更好的机械性能。因此,对在该ph下制备的膜进行了后续实验。对膜的阻隔性能、颜色、抗氧化性能、热稳定性和交联度进行了评价。5u mgase的掺入导致薄膜基体内随机交联形成,这对薄膜的机械性能和屏障性能产生了负面影响。相比之下,10 U mgase增强了薄膜的物理性能,并产生了更高的交联度。百里香油(thyymus vulgaris)加入成膜液中,以提高其抗氧化和抗菌性能。虽然1%百里香油提高了膜的活性性能,但降低了膜的机械强度、阻隔性能和交联度。尽管存在这些挑战,但建议将1%百里香油与10 U MTGase交联在薄膜中,以开发具有高抗氧化能力和抗菌效果的活性包装膜。
Effects of transglutaminase and thyme oil addition on the properties of faba bean protein-based active films
In this study, faba bean (Vicia faba L.) protein concentrate-based biodegradable films were developed using the casting method at two pH levels (6 and 8) and with different microbial transglutaminase (MTGase) concentrations (0, 5 U, and 10 U). Films prepared at pH 8 exhibited improved mechanical properties due to the higher solubility of proteins. Therefore, subsequent experiments were conducted with the films prepared at this pH. The films were evaluated for their barrier properties, color, antioxidant properties, thermal stability, and degree of cross-linking. The incorporation of 5 U MTGase led to random cross-link formation within the film matrix, which negatively impacted the mechanical and barrier properties. In contrast, 10 U MTGase enhanced the physical properties of the films and resulted in a higher degree of cross-linking. Thyme oil (Thymus vulgaris) was incorporated into the film-forming solutions to enhance their antioxidant and antimicrobial properties. While 1 % thyme oil enhanced the active properties of the films, it reduced their mechanical strength, barrier performance, and degree of cross-linking. Despite these challenges, incorporating 1 % thyme oil in films crosslinked with 10 U MTGase is recommended for developing active packaging films with high antioxidant capacity and antimicrobial effectiveness.
期刊介绍:
The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.