Hui Li , Huanjing Sheng , Jianyun Zhao , Xinhao Zhang , Menghan Li , Lanxin Zhao , Lanqi Li , Xiaoman Zhang , Baowei Yang , Séamus Fanning , Yang Wang , Shaofei Yan , Li Bai
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The majority of acquired MDR isolates (<em>n =</em> 31, 92.3 %) belonged to hypovirulent clonal complex (CC) 9 (Lineage II, IIc), including 3 sequence types (ST) (ST9, <em>n =</em> 29; ST2458, <em>n =</em> 1; ST9-1LV, <em>n =</em> 1), which has remained dominant over the past decade. In 2022, three additional acquired MDR clones emerged: CC87/ST87 (Lineage I, IIb), CC8/ST8 (Lineage II, IIa), and CC155/ST705 (Lineage II, IIa), with CC87/ST87 and CC8/ST8 being notably associated with human listeriosis in Asia. The <em>rep25_2_M640p00130</em> plasmid was the most common mobile genetic element among these acquired MDR isolates, consistently harboring seven types of antibiotic resistance genes, including aminoglycosides (<em>ant</em>(6)<em>-Ia</em>; <em>aph</em>(3′)<em>-III</em>), trimethoprim (<em>dfrG</em>), macrolides, lincosamides and streptogramin B (MLS<sub>b</sub>) (<em>erm</em>(B)), lincosamides (<em>lnu</em>(B)), pleuromutilins, lincosamides and streptogramin A (PLS<sub>A</sub>) (<em>lsa</em>(E)), tetracyclines (<em>tet</em>(S)), and phenicols (<em>catA</em>), and flanked on one side by IS<em>1216E</em>. However, the diversity of acquired MDR-carrying plasmids increased from 2017 to 2022, with an increased prevalence among replicons including <em>rep26_2_repA</em>, <em>rep26_4_repA</em>, and <em>rep26_1_pli0070/rep32_1_pli0023</em>. Importantly, compared to the dominant hypovirulent CC9, which contained premature stop codons in the internalin gene <em>inlA</em> associated with adhesion and invasion, the newly emerged acquired MDR <em>L. monocytogenes</em> CC8/ST8 and CC155/ST705 maintained intact <em>inlA</em> gene and exhibited stronger adhesion and invasion phenotype in Caco-2 cells. These findings emphasize the need for continuous surveillance of acquired MDR <em>L. monocytogenes</em>, particularly the virulent CC8/ST8 and CC155/ST705, to mitigate risks to food safety and human health.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"439 ","pages":"Article 111236"},"PeriodicalIF":5.0000,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Emerging threats: Listeria monocytogenes with acquired multidrug resistance from food in China, 2012–2022\",\"authors\":\"Hui Li , Huanjing Sheng , Jianyun Zhao , Xinhao Zhang , Menghan Li , Lanxin Zhao , Lanqi Li , Xiaoman Zhang , Baowei Yang , Séamus Fanning , Yang Wang , Shaofei Yan , Li Bai\",\"doi\":\"10.1016/j.ijfoodmicro.2025.111236\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div><em>Listeria monocytogenes</em> is a foodborne pathogen that poses threat to food safety and public health. 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引用次数: 0
摘要
单核增生李斯特菌是一种威胁食品安全和公众健康的食源性致病菌。一般来说,单核增生乳杆菌对临床重要抗生素的耐药率很低。本研究旨在调查2012 - 2022年中国食品样品中获得性多药耐药(MDR)单核增生乳杆菌的流行情况及遗传特征。在收集到的8344株菌株中,34株(0.41%)被鉴定为获得性MDR。大多数获得性MDR分离株(n = 31, 92.3%)属于低毒性克隆复合体(CC) 9(谱系II, IIc),包括3种序列类型(ST) (ST9, n = 29;ST2458, n = 1;ST9-1LV, n = 1),在过去十年中一直占据主导地位。2022年,又出现了三个获得的MDR克隆:CC87/ST87 (Lineage I, IIb)、CC8/ST8 (Lineage II, IIa)和CC155/ST705 (Lineage II, IIa),其中CC87/ST87和CC8/ST8与亚洲人类李斯特菌病明显相关。在这些获得的MDR分离株中,rep25_2_M640p00130质粒是最常见的移动遗传元件,始终包含7种抗生素耐药基因,包括氨基糖苷类(ant(6)-Ia;图(3’)-III)、甲氧苄氨嘧啶(dfrG)、大环内酯类、lincosamides和链霉素B (MLSb) (erm(B))、lincosamides (lnu(B))、pleuromultilins、lincosamides和链霉素A (PLSA) (lsa(E))、四环素(tet(S))和酚类(catA),并在一侧被IS1216E包围。然而,从2017年到2022年,获得性携带耐多药质粒的多样性有所增加,rep26_2_repA、rep26_4_repA和rep26_1_pli0070/rep32_1_pli0023等复制子的患病率增加。重要的是,与显性低毒CC9相比,新出现的获得性单核细胞增生多耐药L. CC8/ST8和CC155/ST705保持了完整的inlA基因,在Caco-2细胞中表现出更强的粘附和侵袭表型。这些发现强调需要持续监测获得性耐多药单核细胞增生乳杆菌,特别是毒性的CC8/ST8和CC155/ST705,以减轻对食品安全和人类健康的风险。
Emerging threats: Listeria monocytogenes with acquired multidrug resistance from food in China, 2012–2022
Listeria monocytogenes is a foodborne pathogen that poses threat to food safety and public health. Generally, the rates of resistance to clinically important antibiotics in L. monocytogenes are low. This study aimed to investigate the prevalence and genetic characteristics of L. monocytogenes with acquired multidrug resistance (MDR) in food samples from China between 2012 and 2022. Of 8344 isolates collected, 34 (0.41 %) were identified as acquired MDR. The majority of acquired MDR isolates (n = 31, 92.3 %) belonged to hypovirulent clonal complex (CC) 9 (Lineage II, IIc), including 3 sequence types (ST) (ST9, n = 29; ST2458, n = 1; ST9-1LV, n = 1), which has remained dominant over the past decade. In 2022, three additional acquired MDR clones emerged: CC87/ST87 (Lineage I, IIb), CC8/ST8 (Lineage II, IIa), and CC155/ST705 (Lineage II, IIa), with CC87/ST87 and CC8/ST8 being notably associated with human listeriosis in Asia. The rep25_2_M640p00130 plasmid was the most common mobile genetic element among these acquired MDR isolates, consistently harboring seven types of antibiotic resistance genes, including aminoglycosides (ant(6)-Ia; aph(3′)-III), trimethoprim (dfrG), macrolides, lincosamides and streptogramin B (MLSb) (erm(B)), lincosamides (lnu(B)), pleuromutilins, lincosamides and streptogramin A (PLSA) (lsa(E)), tetracyclines (tet(S)), and phenicols (catA), and flanked on one side by IS1216E. However, the diversity of acquired MDR-carrying plasmids increased from 2017 to 2022, with an increased prevalence among replicons including rep26_2_repA, rep26_4_repA, and rep26_1_pli0070/rep32_1_pli0023. Importantly, compared to the dominant hypovirulent CC9, which contained premature stop codons in the internalin gene inlA associated with adhesion and invasion, the newly emerged acquired MDR L. monocytogenes CC8/ST8 and CC155/ST705 maintained intact inlA gene and exhibited stronger adhesion and invasion phenotype in Caco-2 cells. These findings emphasize the need for continuous surveillance of acquired MDR L. monocytogenes, particularly the virulent CC8/ST8 and CC155/ST705, to mitigate risks to food safety and human health.
期刊介绍:
The International Journal of Food Microbiology publishes papers dealing with all aspects of food microbiology. Articles must present information that is novel, has high impact and interest, and is of high scientific quality. They should provide scientific or technological advancement in the specific field of interest of the journal and enhance its strong international reputation. Preliminary or confirmatory results as well as contributions not strictly related to food microbiology will not be considered for publication.