革命性的神经再生：聚乳酸/壳聚糖管道与神经样细胞和假马齿苋在雄性大鼠模型中的应用

IF 3.4 3区环境科学与生态学 Q3 CELL & TISSUE ENGINEERING

Regenerative Therapy Pub Date : 2025-05-15 DOI:10.1016/j.reth.2025.05.003

Elham Hoveizi , Hadi Naddaf , Emad Naeimavi

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引用次数: 0

摘要

目的将子宫内膜间充质干细胞（EnMSCs）衍生的神经样细胞（NLCs）与聚乳酸/壳聚糖支架（PLA/CS）结合使用假马齿草（Memocopa）构建大鼠坐骨神经损伤模型，实现坐骨神经再生。虽然之前的研究已经探索了干细胞疗法和基于支架的神经再生方法，但将EnMSCs与PLA/CS支架和Memocopa结合使用代表了一种新颖的、潜在的协同方法。方法在一项研究中分离、表征和分化mscs。利用RT-PCR和免疫细胞化学方法证实了分化细胞中特异性基因的表达。制备了用于神经组织工程的聚乳酸纳米纤维和壳聚糖水凝胶支架。Memocopa与支架和细胞移植一起口服。该研究涉及25只成年雄性Wistar大鼠，坐骨神经间隙为3毫米，根据治疗分为五组。动物监测8周，期间测量SFI。然后准备组织样本进行组织学检查，包括各种染色技术。结果支架、细胞和Memocopa联合使用对坐骨神经功能有显著改善，第8周SFI结果：导管组- 33.87，导管/细胞组- 25.92，导管/细胞/Memocopa组- 22.86，Memocopa组- 30.93，对照组- 38.87。组织学结果显示各方面均有改善，包括不同治疗组的新神经形成百分比：导管组4.62%，导管/细胞组16.45%，导管/细胞/Memocopa组21.32%，Memocopa组7.07%，对照组0.22%。结论本研究结果表明，EnMSCs向NLCs的高效分化是可能的，PLA/CS支架和Memocopa同时使用可以修复和改善大鼠坐骨神经损伤动物模型。

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Revolutionizing nerve regeneration: A novel approach using polylactic acid/chitosan conduit with nerve-like cells and Bacopa monnieri in male rat model

Objectives

This research aims to use neural-like cells (NLCs) derived from endometrial mesenchymal stem cells (EnMSCs) on a polylactic acid/chitosan scaffold (PLA/CS) along with the use of bacopa monnieri (Memocopa) in a rat sciatic nerve injury model for sciatic nerve regeneration. While previous studies have explored stem cell therapies and scaffold-based approaches for nerve regeneration, using EnMSCs in combination with a PLA/CS scaffold and Memocopa represents a novel, potentially synergistic approach.

Method

EnMSCs were isolated, characterized, and differentiated in a study. The expression of specific genes in the differentiated cells was confirmed using RT-PCR and immunocytochemistry. PLA nanofiber and chitosan hydrogel scaffolds were created for neural tissue engineering. Memocopa was administered orally alongside scaffold and cell transplantation. The study involved 25 adult male Wistar rats with a 3 mm sciatic nerve gap, divided into five groups based on treatment. Animals were monitored for 8 weeks, during which SFI was measured. Tissue samples were then prepared for histological examination, including various staining techniques.

Results

The combination of scaffold, cells, and Memocopa showed significant improvements in sciatic nerve function, as indicated by the SFI results in the eighth week: conduit group −33.87, conduit/cells group −25.92, conduit/cells/Memocopa group −22.86, Memocopa group −30.93, and control group −38.87. Histological findings revealed improvements in various aspects, including percentages of new nerve formation across the different treatment groups: conduit group 4.62 %, conduit/cells group 16.45 %, conduit/cells/Memocopa group 21.32 %, Memocopa group 7.07 %, and control group 0.22 %.

Conclusions

The results of this study showed that efficient differentiation of EnMSCs into NLCs is possible, and with the help of PLA/CS scaffold and simultaneous use of Memocopa, it is possible to repair and improve sciatic nerve injury in a rat animal model.

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来源期刊

Regenerative Therapy Engineering-Biomedical Engineering

CiteScore

6.00

自引率

2.30%

发文量

106

审稿时长

49 days

期刊介绍： Regenerative Therapy is the official peer-reviewed online journal of the Japanese Society for Regenerative Medicine. Regenerative Therapy is a multidisciplinary journal that publishes original articles and reviews of basic research, clinical translation, industrial development, and regulatory issues focusing on stem cell biology, tissue engineering, and regenerative medicine.