等离子体处理、聚多巴胺涂层和氯己定三改性PEEK表面：生物相容性和抗菌性能的评估

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials Pub Date : 2025-04-17 DOI:10.1016/j.dental.2025.04.004

Tao Yang , Yuan Zhang , Zhenhua Gao , Beibei Li

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引用次数: 0

摘要

目的研究聚醚醚酮（PEEK）表面冷等离子体处理与多巴胺交联氯己定（CHX）相结合的新型表面修饰策略，以提高其软组织整合和抗菌性能，并为其在牙科领域的应用奠定基础。方法采用冷等离子体处理、聚多巴胺包被和CHX功能化对speek表面进行修饰。通过SEM， FTIR， XPS，轮廓仪和接触角测量对表面进行了表征。进行了15天的CHX体外释放试验，以评估修饰表面的给药特性。通过人牙龈成纤维细胞（HGFs）培养，检测细胞粘附和增殖能力来评估生物反应。通过细菌活力、菌落计数、生物膜形成和粘附分析系统地评估抗菌效果。结果P-PDA-CHX改性显著提高了表面亲水性，使接触角从83.6 ± 3.1°降低到24.2 ± 4.2°。p - pda - chx组的表面粗糙度（0.38 ± 0.029 μm）显著高于p - pda组（0.28 ± 0.048 μm）和p - chx组（0.33 ± 0.033 μm）（p <； 0.05）。与未修饰的表面相比，修饰的表面增强了HGFs的增殖和粘附，细胞表现出更好的扩散和细胞骨架组织。P-PDA-CHX涂层在15天内持续释放氯己定，并表现出优异的抗菌性能，显著减少细菌粘附和生物膜的形成，同时与hgf保持良好的生物相容性。这种表面改性策略为提高PEEK的生物和抗菌性能提供了一条很有前途的途径。冷等离子体处理、PDA涂层和CHX功能化的结合提供了一种增强细胞反应和细菌耐药性的解决方案，这可以使PEEK材料的各种牙科应用受益，包括定制基台，其中组织整合和细菌控制是必不可少的。

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Triple-modified PEEK surface via plasma treatment, polydopamine coating and chlorhexidine: Assessment of biocompatibility and antibacterial properties

Objective

To develop and evaluate a novel surface modification strategy for polyetheretherketone (PEEK) that combines cold plasma treatment with dopamine-crosslinked chlorhexidine (CHX) to enhance both soft tissue integration and antimicrobial properties for potential dental applications.

Methods

PEEK surfaces were modified through cold plasma treatment followed by polydopamine (PDA) coating and CHX functionalization. Surface characterization was performed by SEM, FTIR, XPS, profilometer and contact angle measurements. A 15-day CHX in vitro release test was conducted to evaluate the drug delivery profile of the modified surfaces. Biological response was evaluated through human gingival fibroblasts (HGFs) cultures, examining cell adhesion and proliferation capacity. Antimicrobial efficacy was systematically evaluated through bacterial viability, colony enumeration, biofilm formation, and adhesion analysis.

Results

The P-PDA-CHX modification significantly improved surface hydrophilicity, reducing contact angles from 83.6 ± 3.1° to 24.2 ± 4.2°. Surface roughness of P-PDA-CHX (0.38 ± 0.029 μm) was significantly higher than that of the P-PDA (0.28 ± 0.048 μm) and P-CHX (0.33 ± 0.033 μm) groups (p < 0.05). Compared to unmodified surfaces, the modified surfaces demonstrated enhanced HGFs proliferation and adhesion, with cells showing improved spreading and cytoskeletal organization. The P-PDA-CHX coating exhibited sustained chlorhexidine release over 15 days and demonstrated superior antibacterial properties, significantly reducing bacterial adhesion and biofilm formation while maintaining excellent biocompatibility with HGFs.

Significance

This surface modification strategy offers a promising approach for improving PEEK's biological and antibacterial properties. The combination of cold plasma treatment, PDA coating, and CHX functionalization provides a solution to enhance both cellular response and bacterial resistance, which could benefit various dental applications of PEEK materials, including customized abutments, where tissue integration and bacterial control are essential.

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来源期刊

Dental Materials 工程技术-材料科学：生物材料

CiteScore

9.80

自引率

10.00%

发文量

290

审稿时长

67 days

期刊介绍： Dental Materials publishes original research, review articles, and short communications. Academy of Dental Materials members click here to register for free access to Dental Materials online. The principal aim of Dental Materials is to promote rapid communication of scientific information between academia, industry, and the dental practitioner. Original Manuscripts on clinical and laboratory research of basic and applied character which focus on the properties or performance of dental materials or the reaction of host tissues to materials are given priority publication. Other acceptable topics include application technology in clinical dentistry and dental laboratory technology. Comprehensive reviews and editorial commentaries on pertinent subjects will be considered.