水分状况改变了金属污染水稻土N2O排放的微生物机制

IF 6.2 2区 环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES
Yifan Guo , Shulan Cheng , Huajun Fang , Jing Geng , Fangying Shi , Hui Wang , Long Chen , Haiguang Pu , Bingqian Liu , Yi Zhou
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引用次数: 0

摘要

微生物通过参与关键的氮(N)相关过程,对土壤氧化亚氮(N2O)排放至关重要。然而,水制度对金属污染土壤中N2O排放与微生物过程之间相互作用的影响尚不清楚。在这里,我们进行了土壤微观环境试验,采用两种水管理策略(不淹水和淹水)和六种金属添加处理,包括低(2和200 mg kg−1)和高(10和1000 mg kg−1)水平的单个和组合Cd和Cu。高水平镉和铜污染对土壤N2O排放的影响因水状况而异,在非淹水条件下表现为拮抗效应,在淹水条件下表现为协同效应。高水平的共污染显著抑制了两种水质下的硝化作用,主要是由于亚硝化螺旋体丰度的减少。相比之下,这种共污染降低了Ramlibacter的丰度,从而抑制了洪水条件下的反硝化和异化硝酸盐还原为铵(DNRA)。抑制这些关键微生物及其介导的n循环过程减少了两种水状态下的土壤N2O排放。这种减少在洪水条件下更大,因为更多的n相关过程被抑制。宏基因组分析进一步表明,亚硝化螺旋体携带硝化基因,拉姆利杆菌携带反硝化、同化硝态氮还原为铵(ANRA)和DNRA相关基因。这些发现表明这两种微生物都有产生N2O的潜力。总体而言,水管理策略和金属污染改变了N2O排放的微生物过程,突出了适当的水管理对减少中国南方金属污染水稻土温室气体排放的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Water regime alters microbial mechanisms of N2O emission in metal-contaminated paddy soils
Microorganisms are essential for soil nitrous oxide (N2O) emissions through participating in key nitrogen (N)-related processes. However, the effect of water regimes on the interactions between N2O emissions and microbial processes in metal-contaminated soils is unclear. Here, we conducted a soil microcosm experiment with two water management strategies (non-flooding and flooding) and six metal addition treatments including low (2 and 200 mg kg−1) and high (10 and 1000 mg kg−1) levels of individual and combined Cd and Cu. The effects of high levels of individual Cd and Cu contamination on soil N2O emissions varied depending on water regimes, showing antagonistic effects under non-flooding conditions and synergistic effects under flooding conditions. High levels of co-contamination significantly inhibited nitrification under both water regimes, primarily due to reduced abundance of Nitrosospira. In contrast, this co-contamination decreased the abundance of Ramlibacter, thereby inhibiting denitrification and dissimilatory nitrate reduction to ammonium (DNRA) under flooding conditions. The inhibition of these key microorganisms and their mediated N-cycle processes reduced soil N2O emissions under both water regimes. This reduction was greater under flooding conditions because more N-related processes were inhibited. Metagenomic binning further indicated that Nitrosospira carried nitrifying genes, while Ramlibacter contained genes involved in denitrification, assimilatory nitrate reduction to ammonium (ANRA), and DNRA. These findings implied that both microorganisms had potential to produce N2O. Overall, water management strategies and metal contamination altered the microbial processes of N2O emissions, highlighting the importance of appropriate water management in mitigating greenhouse gas emissions from metal-contaminated paddy soils in southern China.
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来源期刊
CiteScore
12.10
自引率
5.90%
发文量
1234
审稿时长
88 days
期刊介绍: Ecotoxicology and Environmental Safety is a multi-disciplinary journal that focuses on understanding the exposure and effects of environmental contamination on organisms including human health. The scope of the journal covers three main themes. The topics within these themes, indicated below, include (but are not limited to) the following: Ecotoxicology、Environmental Chemistry、Environmental Safety etc.
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