巴豆绿银纳米颗粒对新热带钉螺的遗传毒性、诱变性和免疫毒性评价

IF 7.3 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Pollution Pub Date : 2025-05-13 DOI:10.1016/j.envpol.2025.126424

Paula Sampaio Araújo , Maxwell Batista Caixeta , Gabriel Qualhato , Bruno Bastos Gonçalves , Rodrigo Saar Gomes , Eloiza da Silva Nunes Viali , Wesley Renato Viali , Thiago Lopes Rocha

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引用次数: 0

摘要

绿色银纳米粒子（G-Ag NPs）已被证明是纳米医学中更安全的纳米技术，特别是在控制医学上重要的微生物、媒介和腹足类动物方面。然而，它们在水生生物中的作用机制和生态毒性尚不清楚。因此，本研究旨在研究巴豆叶水提物合成的G-Ag NPs对新热带淡水蜗牛光螺（Biomphalaria glabrata）体内暴露后的影响。将成螺分别暴露于G-Ag NPs（0.05和0.14 mg L-1）或G-Ag水提物（5.4 mg L-1）中7 d。在血细胞中分析遗传毒性（彗星试验）、致突变性（微核试验和核异常试验）和免疫毒性（流式细胞术吞噬试验）。结果表明，G-Ag NPs与叶片水提液和阴性对照组相比，具有较高的死亡率和基因毒性（DNA损伤）。G-Ag NPs也诱导诱变效应（细胞核改变），主要是细胞核的缺口和泡状。G-Ag NPs和提取物对血细胞的吞噬活性和细胞活力没有影响。这些发现有助于了解绿色纳米颗粒在淡水腹足动物中的毒性，并代表了评估G-Ag NPs和C. urucurana水提取物对光斑贝血细胞的影响的开创性努力。总的来说，腹足类动物的免疫系统是绿色纳米颗粒毒性的目标。

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Genotoxicity, mutagenicity, and immunotoxicity assessment of green silver nanoparticles from Croton urucurana on neotropical snails Biomphalaria glabrata

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Genotoxicity, mutagenicity, and immunotoxicity assessment of green silver nanoparticles from Croton urucurana on neotropical snails Biomphalaria glabrata

Green silver nanoparticles (G-Ag NPs) have been indicated as safer nanotechnology in nanomedicine, especially for controlling microorganisms, vectors, and gastropods of medical importance. However, their mechanisms of action and ecotoxicity in aquatic organisms remain unknown. Thus, the current study aimed to investigate the effects of G-Ag NPs synthesized through Croton urucurana leave aqueous extract on the neotropical freshwater snail Biomphalaria glabrata after in vivo exposure using multi-biomarker responses in hemocytes. Adult snails were exposed to G-Ag NPs (0.05 and 0.14 mg L⁻¹) or leave aqueous extract (5.4 mg L⁻¹) for 7 days. Genotoxicity (comet assay), mutagenicity (micronucleus test and nuclear abnormality assay), and immunotoxicity (phagocytosis assay by flow cytometry) were analyzed in hemocytes. Results showed that G-Ag NPs induced high mortality and genotoxic effects (DNA damage) compared to leave aqueous extract and negative control groups. G-Ag NPs also induced mutagenic effects (nuclear alterations), mainly notched and blebbed nuclei. Also, G-Ag NPs and extract did not change the phagocytic activity and cell viability of hemocytes. These findings contribute to understanding the toxicity of green nanoparticles in freshwater gastropods and represent a pioneering effort to evaluate the effects of G-Ag NPs and the C. urucurana aqueous extract in B. glabrata hemocytes. Overall, the immune system of gastropods is a target of green nanoparticle toxicity.

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来源期刊

Environmental Pollution 环境科学-环境科学

CiteScore

16.00

自引率

6.70%

发文量

2082

审稿时长

2.9 months

期刊介绍： Environmental Pollution is an international peer-reviewed journal that publishes high-quality research papers and review articles covering all aspects of environmental pollution and its impacts on ecosystems and human health. Subject areas include, but are not limited to: • Sources and occurrences of pollutants that are clearly defined and measured in environmental compartments, food and food-related items, and human bodies; • Interlinks between contaminant exposure and biological, ecological, and human health effects, including those of climate change; • Contaminants of emerging concerns (including but not limited to antibiotic resistant microorganisms or genes, microplastics/nanoplastics, electronic wastes, light, and noise) and/or their biological, ecological, or human health effects; • Laboratory and field studies on the remediation/mitigation of environmental pollution via new techniques and with clear links to biological, ecological, or human health effects; • Modeling of pollution processes, patterns, or trends that is of clear environmental and/or human health interest; • New techniques that measure and examine environmental occurrences, transport, behavior, and effects of pollutants within the environment or the laboratory, provided that they can be clearly used to address problems within regional or global environmental compartments.