{"title":"puubl5介导的锌指蛋白1的稳定性对干旱胁迫下乌苏杨根系发育至关重要。","authors":"Haoqin Zhao,Yanrui Fu,Wanqiu Lv,Xin Zhang,Jingjing Li,Da Yang,Lin Shi,Hanzeng Wang,Wanxin Li,Haijiao Huang,Shicheng Zhao,Chenghao Li,Jingli Yang","doi":"10.1093/plphys/kiaf181","DOIUrl":null,"url":null,"abstract":"C2H2-type zinc finger protein (ZFP) transcription factors influence root growth and development. However, their potential roles in inhibiting adventitious root (AR) and lateral root (LR) formation in trees remain unclear. Here, we report that the ABA-responsive C2H2-type zinc finger protein transcription factor (PuZFP1) regulates Populus ussuriensis root development to enhance drought tolerance. PuZFP1 negatively regulates LR development by binding to the PuWRKY46 promoter and inhibiting its expression. At the same time, PuZFP1 promotes AR elongation by repressing Clade E Growth-Regulating (EGR) Type 2C protein phosphatases (PuEGR1). In PuZFP1-overexpressing lines, a higher ABA/IAA ratio in the differentiation zone (DZ) drives PuWRKY46-mediated LR inhibition. Conversely, a lower ABA/IAA ratio is associated with AR elongation and the expression of the downstream target gene PuEGR1 in the elongation zone (EZ). Notably, PuZFP1 physically interacts with Ubiquitin-like protein 5 (PuUBL5) and undergoes 26S proteasome-mediated degradation. Taken together, our findings shed light on the role of the PuUBL5-PuZFP1 module in mediating the crosstalk between LR emergence and AR elongation via ABA/auxin signaling in drought-stressed P. ussuriensis, and provide insights into the regulatory network underlying PuZFP1-mediated root growth in poplar.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"13 1","pages":""},"PeriodicalIF":6.5000,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"PuUBL5-mediated ZINC FINGER PROTEIN 1 stability is critical for root development under drought stress in Populus ussuriensis.\",\"authors\":\"Haoqin Zhao,Yanrui Fu,Wanqiu Lv,Xin Zhang,Jingjing Li,Da Yang,Lin Shi,Hanzeng Wang,Wanxin Li,Haijiao Huang,Shicheng Zhao,Chenghao Li,Jingli Yang\",\"doi\":\"10.1093/plphys/kiaf181\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"C2H2-type zinc finger protein (ZFP) transcription factors influence root growth and development. However, their potential roles in inhibiting adventitious root (AR) and lateral root (LR) formation in trees remain unclear. Here, we report that the ABA-responsive C2H2-type zinc finger protein transcription factor (PuZFP1) regulates Populus ussuriensis root development to enhance drought tolerance. PuZFP1 negatively regulates LR development by binding to the PuWRKY46 promoter and inhibiting its expression. At the same time, PuZFP1 promotes AR elongation by repressing Clade E Growth-Regulating (EGR) Type 2C protein phosphatases (PuEGR1). In PuZFP1-overexpressing lines, a higher ABA/IAA ratio in the differentiation zone (DZ) drives PuWRKY46-mediated LR inhibition. Conversely, a lower ABA/IAA ratio is associated with AR elongation and the expression of the downstream target gene PuEGR1 in the elongation zone (EZ). Notably, PuZFP1 physically interacts with Ubiquitin-like protein 5 (PuUBL5) and undergoes 26S proteasome-mediated degradation. Taken together, our findings shed light on the role of the PuUBL5-PuZFP1 module in mediating the crosstalk between LR emergence and AR elongation via ABA/auxin signaling in drought-stressed P. ussuriensis, and provide insights into the regulatory network underlying PuZFP1-mediated root growth in poplar.\",\"PeriodicalId\":20101,\"journal\":{\"name\":\"Plant Physiology\",\"volume\":\"13 1\",\"pages\":\"\"},\"PeriodicalIF\":6.5000,\"publicationDate\":\"2025-05-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Physiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1093/plphys/kiaf181\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1093/plphys/kiaf181","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
PuUBL5-mediated ZINC FINGER PROTEIN 1 stability is critical for root development under drought stress in Populus ussuriensis.
C2H2-type zinc finger protein (ZFP) transcription factors influence root growth and development. However, their potential roles in inhibiting adventitious root (AR) and lateral root (LR) formation in trees remain unclear. Here, we report that the ABA-responsive C2H2-type zinc finger protein transcription factor (PuZFP1) regulates Populus ussuriensis root development to enhance drought tolerance. PuZFP1 negatively regulates LR development by binding to the PuWRKY46 promoter and inhibiting its expression. At the same time, PuZFP1 promotes AR elongation by repressing Clade E Growth-Regulating (EGR) Type 2C protein phosphatases (PuEGR1). In PuZFP1-overexpressing lines, a higher ABA/IAA ratio in the differentiation zone (DZ) drives PuWRKY46-mediated LR inhibition. Conversely, a lower ABA/IAA ratio is associated with AR elongation and the expression of the downstream target gene PuEGR1 in the elongation zone (EZ). Notably, PuZFP1 physically interacts with Ubiquitin-like protein 5 (PuUBL5) and undergoes 26S proteasome-mediated degradation. Taken together, our findings shed light on the role of the PuUBL5-PuZFP1 module in mediating the crosstalk between LR emergence and AR elongation via ABA/auxin signaling in drought-stressed P. ussuriensis, and provide insights into the regulatory network underlying PuZFP1-mediated root growth in poplar.
期刊介绍:
Plant Physiology® is a distinguished and highly respected journal with a rich history dating back to its establishment in 1926. It stands as a leading international publication in the field of plant biology, covering a comprehensive range of topics from the molecular and structural aspects of plant life to systems biology and ecophysiology. Recognized as the most highly cited journal in plant sciences, Plant Physiology® is a testament to its commitment to excellence and the dissemination of groundbreaking research.
As the official publication of the American Society of Plant Biologists, Plant Physiology® upholds rigorous peer-review standards, ensuring that the scientific community receives the highest quality research. The journal releases 12 issues annually, providing a steady stream of new findings and insights to its readership.