分裂基因编码钙指示细胞器间连接。

IF 9.1 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2025-05-13 DOI:10.1073/pnas.2415268122

Shunit Olszakier,Wessal Hussein,Ronit Heinrich,Michael Andreyanov,Achinoam Blau,Yara Otor,Jackie Schiller,Shai Kellner,Shai Berlin

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引用次数: 0

摘要

基因编码钙指标（GECIs）已经彻底改变了细胞钙信号的研究，为钙动态的实时光学监测提供了强大的工具。虽然当代的GECIs可以靶向各种细胞器，但没有办法在细胞器间连接处获得活性和功能性的GECIs。为了解决这一差距，我们开发了一个工具箱，其中包含绿色和红色geci的分裂版本，旨在仅在两个“半”接近时才重新组装。我们开发了分裂探针来研究线粒体和内质网之间（通过split- megic）或质膜和内质网之间（通过split-sf- member）的细胞器间连通性和活性。我们使用各种分裂传感器在体外和体内成像神经Ca2+活性，并在此过程中，通过使用分裂- megic识别Mito-ER连接和单个树突棘内的钙活性。

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Split genetically encoded calcium indicators for interorganellar junctions.

Genetically encoded calcium indicators (GECIs) have revolutionized the study of cellular calcium signaling, offering powerful tools for real-time optical monitoring of calcium dynamics. Although contemporary GECIs can be targeted to various organelles, there are no means to obtain active and functional GECIs exclusively at interorganellar junctions. To address this gap, we have developed a toolbox of split versions of green and red GECIs designed to reassemble only when the two "halves" come into proximity. We developed split probes to investigate interorganellar connectivity and activity between mitochondria and the ER (via split-MEGIC) or between the plasma membrane and the ER (via split-sf-MEMBER). We employ the various split-sensors to image neural Ca2+ activity in vitro and in vivo and, in the process, identify Mito-ER junctions and calcium activity within individual dendritic spines by use of split-MEGIC.

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来源期刊

Proceedings of the National Academy of Sciences of the United States of America 综合性期刊-综合性期刊

CiteScore

19.00

自引率

0.90%

发文量

3575

审稿时长

2.5 months

期刊介绍： The Proceedings of the National Academy of Sciences (PNAS), a peer-reviewed journal of the National Academy of Sciences (NAS), serves as an authoritative source for high-impact, original research across the biological, physical, and social sciences. With a global scope, the journal welcomes submissions from researchers worldwide, making it an inclusive platform for advancing scientific knowledge.