{"title":"aoac法2017.16：低分子量可溶性膳食纤维低聚糖的检测","authors":"Rebekka Elke Schmidt, Mirko Bunzel","doi":"10.1002/cche.10885","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background and Objectives</h3>\n \n <p>In many countries, the Codex Alimentarius definition of dietary fiber has been adopted with the optional inclusion of oligosaccharides with a degree of polymerization (dp) of ≥ 3. These oligosaccharides are usually captured as low molecular weight soluble dietary fiber (LMWSDF). The suitability of the AOAC-method 2017.16 for the determination of LMWSDF was evaluated, focusing on the correct differentiation between LMWSDF and mono- and disaccharides using different groups of oligosaccharides.</p>\n </section>\n \n <section>\n \n <h3> Findings</h3>\n \n <p>Desalting of LMWSDF with ion exchange resins resulted in an expected, almost complete loss of uronic acid-based oligosaccharides. Due to their elution behavior in size exclusion chromatography, pentotrioses (arabinotriose, xylotriose) were excluded from LMWSDF. Differently, 1,6-linked hexobioses such as melibiose eluted earlier than 1,4-linked hexobioses (such as the standard maltose) and were incorrectly captured as LMWSDF.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>A precise determination of LMWSDF content using AOAC-method 2017.16 depends on the sample to be analyzed. As LMWSDF cannot always be analyzed correctly, these data raise additional questions about the suitability of the dietary fiber definition.</p>\n </section>\n \n <section>\n \n <h3> Significance and Novelty</h3>\n \n <p>This study indicates that data of the AOAC-method 2017.16 applied to samples containing rather unusual oligosaccharides have to be carefully interpreted.</p>\n </section>\n </div>","PeriodicalId":9807,"journal":{"name":"Cereal Chemistry","volume":"102 3","pages":"431-437"},"PeriodicalIF":2.2000,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cche.10885","citationCount":"0","resultStr":"{\"title\":\"Evaluation of AOAC-Method 2017.16: Detection of Oligosaccharides as Low Molecular Weight Soluble Dietary Fiber\",\"authors\":\"Rebekka Elke Schmidt, Mirko Bunzel\",\"doi\":\"10.1002/cche.10885\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background and Objectives</h3>\\n \\n <p>In many countries, the Codex Alimentarius definition of dietary fiber has been adopted with the optional inclusion of oligosaccharides with a degree of polymerization (dp) of ≥ 3. These oligosaccharides are usually captured as low molecular weight soluble dietary fiber (LMWSDF). The suitability of the AOAC-method 2017.16 for the determination of LMWSDF was evaluated, focusing on the correct differentiation between LMWSDF and mono- and disaccharides using different groups of oligosaccharides.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Findings</h3>\\n \\n <p>Desalting of LMWSDF with ion exchange resins resulted in an expected, almost complete loss of uronic acid-based oligosaccharides. Due to their elution behavior in size exclusion chromatography, pentotrioses (arabinotriose, xylotriose) were excluded from LMWSDF. Differently, 1,6-linked hexobioses such as melibiose eluted earlier than 1,4-linked hexobioses (such as the standard maltose) and were incorrectly captured as LMWSDF.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>A precise determination of LMWSDF content using AOAC-method 2017.16 depends on the sample to be analyzed. As LMWSDF cannot always be analyzed correctly, these data raise additional questions about the suitability of the dietary fiber definition.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Significance and Novelty</h3>\\n \\n <p>This study indicates that data of the AOAC-method 2017.16 applied to samples containing rather unusual oligosaccharides have to be carefully interpreted.</p>\\n </section>\\n </div>\",\"PeriodicalId\":9807,\"journal\":{\"name\":\"Cereal Chemistry\",\"volume\":\"102 3\",\"pages\":\"431-437\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-04-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cche.10885\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Cereal Chemistry\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/cche.10885\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cereal Chemistry","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cche.10885","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
Evaluation of AOAC-Method 2017.16: Detection of Oligosaccharides as Low Molecular Weight Soluble Dietary Fiber
Background and Objectives
In many countries, the Codex Alimentarius definition of dietary fiber has been adopted with the optional inclusion of oligosaccharides with a degree of polymerization (dp) of ≥ 3. These oligosaccharides are usually captured as low molecular weight soluble dietary fiber (LMWSDF). The suitability of the AOAC-method 2017.16 for the determination of LMWSDF was evaluated, focusing on the correct differentiation between LMWSDF and mono- and disaccharides using different groups of oligosaccharides.
Findings
Desalting of LMWSDF with ion exchange resins resulted in an expected, almost complete loss of uronic acid-based oligosaccharides. Due to their elution behavior in size exclusion chromatography, pentotrioses (arabinotriose, xylotriose) were excluded from LMWSDF. Differently, 1,6-linked hexobioses such as melibiose eluted earlier than 1,4-linked hexobioses (such as the standard maltose) and were incorrectly captured as LMWSDF.
Conclusions
A precise determination of LMWSDF content using AOAC-method 2017.16 depends on the sample to be analyzed. As LMWSDF cannot always be analyzed correctly, these data raise additional questions about the suitability of the dietary fiber definition.
Significance and Novelty
This study indicates that data of the AOAC-method 2017.16 applied to samples containing rather unusual oligosaccharides have to be carefully interpreted.
期刊介绍：
Cereal Chemistry publishes high-quality papers reporting novel research and significant conceptual advances in genetics, biotechnology, composition, processing, and utilization of cereal grains (barley, maize, millet, oats, rice, rye, sorghum, triticale, and wheat), pulses (beans, lentils, peas, etc.), oilseeds, and specialty crops (amaranth, flax, quinoa, etc.). Papers advancing grain science in relation to health, nutrition, pet and animal food, and safety, along with new methodologies, instrumentation, and analysis relating to these areas are welcome, as are research notes and topical review papers.
The journal generally does not accept papers that focus on nongrain ingredients, technology of a commercial or proprietary nature, or that confirm previous research without extending knowledge. Papers that describe product development should include discussion of underlying theoretical principles.
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