封面图：基于连续滴定的非共价相互作用快速溶液分析方法。方法5/2025)

IF 6.1 Q1 CHEMISTRY, MULTIDISCIPLINARY

Chemistry methods : new approaches to solving problems in chemistry Pub Date : 2025-05-13 DOI:10.1002/cmtd.202580501

Philipp Willmer, Adam C. Hundahl, Rodolphe Marie, Henrik Jensen

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引用次数: 0

摘要

封面展示了一种新的方法（cSPRING）来研究暴露于流动毛细管内小配体连续滴定的目标分子的相互作用。目标的荧光发射在两个独立的光谱带中检测。比率测量可以从一次测量中提取结合亲和度（Kd）。只需要两个样品和不到一分钟，这种方法非常适合于高通量筛选应用。要了解更多细节，请参阅Henrik Jensen及其同事的研究文章（10.1002/cmtd.202400059）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Cover Picture: Continuous Titration Based Method for Rapid In-Solution Analysis of Non-Covalent Interactions (Chem. Methods 5/2025)

The Front Cover illustrates a new method (cSPRING) to study the interaction of a target molecule exposed to a continuous titration of a small ligand inside a flow capillary. The fluorescence emission of the target is detected in two separate spectral bands. A ratiometric measurement allows to extract the binding affinity (K_d) from a single measurement. Requiring only two samples and less than a minute, this method is well-suited for high-throughput screening applications. For more details, see the Research Article by Henrik Jensen and co-workers (10.1002/cmtd.202400059).

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来源期刊

Chemistry methods : new approaches to solving problems in chemistry

CiteScore

7.30

自引率

0.00%

发文量