接触印刷法共价DNA固定化工艺的优化

IF 0.7 Q4 CHEMISTRY, MULTIDISCIPLINARY

Moscow University Chemistry Bulletin Pub Date : 2025-05-13 DOI:10.3103/S0027131425700075

A. A. Titov, A. E. Kuznetsov, N. V. Komarova

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引用次数: 0

摘要

接触式印刷方法被广泛用于制造微芯片和开发高灵敏度的生物传感器。这种方法是基于固定化生物分子的技术。本文优化了基于二硫键形成反应和叠氮化物-炔环加成反应的两种有效的寡核苷酸共价固定方法。通过这两种固定策略，寡核苷酸保留了与互补DNA杂交的能力，这为它们在生物分析应用中的实际应用提供了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Optimization of Covalent DNA Immobilization for Contact Printing Method

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Optimization of Covalent DNA Immobilization for Contact Printing Method

The contact printing method is widely used to create microchips and develop highly sensitive biosensors. This method is based on techniques for immobilizing biomolecules. In this work, two effective methods for covalent immobilization of oligonucleotides based on reactions of disulfide bond formation and azide–alkyne cycloaddition are optimized for the contact printing method. With both immobilization strategies, oligonucleotides retain the ability to hybridize with complementary DNA, which provides a basis for their practical use in bioanalytical applications.

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来源期刊

Moscow University Chemistry Bulletin CHEMISTRY, MULTIDISCIPLINARY-

CiteScore

1.30

自引率

14.30%

发文量

期刊介绍： Moscow University Chemistry Bulletin is a journal that publishes review articles, original research articles, and short communications on various areas of basic and applied research in chemistry, including medical chemistry and pharmacology.