一种高灵敏快速检测十足类虹膜病毒1型的DNAzyme生物传感器

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture Pub Date : 2025-05-10 DOI:10.1016/j.aquaculture.2025.742688

Shuai Zhang , Siying Li , Huili Tian , Ming'ao Wang , Mingsheng Lyu , Shujun Wang

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引用次数: 0

摘要

彩虹十足病毒1 （DIV1）导致虾类养殖死亡率高，对水产养殖业造成重大经济影响。目前，没有有效的治疗方法来控制DIV1感染，管理主要依赖于预防措施。DIV1的快速准确诊断至关重要。在本研究中，我们通过体外选择鉴定了两种DNA酶，MCP-1和MCP-8，可以特异性识别DIV1。MCP-1的检出限为DIV1的6.89拷贝/μL。我们通过集成SYBR Green I增强信号，开发了一种基于dnazyme的“关闭”生物传感器，用于快速检测DIV1，该传感器具有高特异性和高灵敏度。生物传感器的检出限可达2.01拷贝/μL。与传统检测方法相比，该传感器在5分钟内提供结果，无需复杂的程序，大大简化了检测过程。

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A DNAzyme biosensor for highly sensitive and rapid detection of decapod iridovirus 1

Decapod Iridescent Virus 1 (DIV1) leads to high mortality rates in shrimp farming, significantly impacting the aquaculture industry economically. Currently, there are no effective treatments for controlling DIV1 infections, and management primarily relies on preventative measures. Rapid and precise diagnosis of DIV1 is crucial. In this study, we identified two DNA enzymes, MCP-1 and MCP-8, that can specifically recognize DIV1 via in vitro selection. The detection limit of MCP-1 was 6.89 copies/μL of DIV1. We developed a DNAzyme-based “turn off” biosensor for quick detection of DIV1, which is both highly specific and sensitive, by integrating SYBR Green I to enhance the signal. The detection limit of biosensor could reach 2.01 copies/μL of DIV1. Compared to conventional detection methods, this sensor delivers results in 5 min without the need for complex procedures, greatly streamlining the detection process.

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来源期刊

Aquaculture 农林科学-海洋与淡水生物学

CiteScore

8.60

自引率

17.80%

发文量

1246

审稿时长

56 days

期刊介绍： Aquaculture is an international journal for the exploration, improvement and management of all freshwater and marine food resources. It publishes novel and innovative research of world-wide interest on farming of aquatic organisms, which includes finfish, mollusks, crustaceans and aquatic plants for human consumption. Research on ornamentals is not a focus of the Journal. Aquaculture only publishes papers with a clear relevance to improving aquaculture practices or a potential application.