液相色谱-串联质谱法同时定量测定抗癫痫药物的干血斑分析

IF 1.8 3区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Rapid Communications in Mass Spectrometry Pub Date : 2025-05-12 DOI:10.1002/rcm.10064

Mariam M. Abady, Ji-Seon Jeong, Ha-Jeong Kwon

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引用次数: 0

摘要

原理干血斑（DBS）取样用于抗癫痫药物的治疗性药物监测具有实际优势，包括最小的侵入性和易于收集。然而，为了精确的治疗管理，其定量的准确性和可靠性有待验证。方法采用液相色谱-串联质谱法（LC-MS/MS）对DBS样品进行分析，克服了DBS样品的物理化学问题。结果乙腈基DBS提取方法对11种抗癫痫药物具有较高的提取效率。准确度和精密度均在6%以内，具有良好的选择性、最小的基质效应和可忽略的残留。所有抗癫痫药物在DBS样品中在室温下表现出至少30天的稳定性，证实了DBS样品的正确处理和储存。从DBS中打出直径3毫米的圆盘，对所有目标药物产生准确的结果。结论优化后的方法具有较好的时间和成本效益，显示了全血中药物浓度之间的强相关性，从而支持DBS取样作为一种有前途的抗癫痫药物监测方法的适用性。

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Dried Blood Spot Analysis for Simultaneous Quantification of Antiepileptic Drugs Using Liquid Chromatography–Tandem Mass Spectrometry

Rationale

Dried blood spot (DBS) sampling for the therapeutic drug monitoring of antiepileptic drugs offers practical advantages, including minimal invasiveness and ease of collection. However, for precise therapeutic management, its accuracy and reliability in quantification need to be validated.

Methods

This study validates DBS sampling for the analysis of 11 antiepileptic drugs using liquid chromatography-tandem mass spectrometry (LC-MS/MS), overcoming the physicochemical challenges associated with DBS samples.

Results

The acetonitrile-based DBS extraction method demonstrated high efficiency for the 11 antiepileptic drugs. Accuracy and precision within 6% were achieved in both intra- and inter-day assays, with good selectivity, minimal matrix effects, and negligible carryover. All antiepileptic drugs exhibited stability in DBS samples for at least 30 days at room temperature, confirming proper handling and storage of the DBS samples. A 3 mm diameter disc punched from a DBS produced accurate results for all target drugs.

Conclusions

The optimized method provided a time- and cost-effective solution, showing a strong correlation between drug concentrations in whole blood, thereby supporting the suitability of DBS sampling as a promising and advanced method for antiepileptic drug monitoring.

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来源期刊

Rapid Communications in Mass Spectrometry 化学-分析化学

CiteScore

4.10

自引率

5.00%

发文量

219

审稿时长

2.6 months

期刊介绍： Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.