G6PD对红细胞质量的影响

IF 1.8 Q3 HEMATOLOGY

Hematology, Transfusion and Cell Therapy Pub Date : 2025-05-13 DOI:10.1016/j.htct.2025.103733

Andrew Evans Cobbinah , Benedict Sackey , Mina Ofosu , Herbert Ekoe Dankluvi , Stephen Opoku , Ampa Davis Frank

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引用次数: 0

摘要

最常见的红细胞代谢状况是葡萄糖-6-磷酸脱氢酶（G6PD）缺乏症，全球约有3500万人受到影响。发病率最高的是东半球的热带和亚热带地区，在那里该病可影响多达35%的人口。G6PD缺乏症是最普遍的酶缺乏症，目前还没有在血液制品中进行检测。G6PD缺乏症是一种影响储存红细胞质量的遗传因素，影响其对氧化应激的反应能力。这项以医院为基础的横断面研究旨在评估供体血液中G6PD缺乏症的患病率以及酶缺乏症对储存期间红细胞指数的影响。方法对57例血袋进行G6PD缺乏筛查。在贮藏第0天、第7天和第14天测定红细胞指数和血膜评价。结果57例患者G6PD完全缺损8例（14%），无缺损86例（49/57）。在14天的储存过程中，与基线和正常G6PD活性相比，G6PD缺陷血单位的血红蛋白和红细胞计数显著降低，相应的平均红细胞体积和红细胞分布宽度-标准差显著增加。在储存2周后，g6pd非缺陷供者的血膜评论显示，85.7%的供者呈正性正色，2.0%的供者呈小细胞低色，12.2%的供者呈大细胞高色，而g6pd缺陷供者的正性正色为75%，12.5%的供者呈小细胞低色，12.5%的供者呈大细胞低色。结论g6pd缺陷供体单位在储存期间红细胞计数和血红蛋白显著减少，平均红细胞体积增加，表明细胞膜稳态机制逐渐丧失，可能导致长期储存期间进一步溶血。

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Blood storage effect of G6PD on RBC quality

Background

The most prevalent metabolic condition of red blood cells, glucose-6-phosphate dehydrogenase (G6PD) deficiency, affects around 35 million people globally. The highest prevalence is seen in tropical and subtropical areas of the eastern hemisphere, where it can affect up to 35 % of the population. G6PD deficiency, the most prevalent enzyme deficit, is not currently tested for in blood products. G6PD deficiency is a genetic factor that influences the quality of stored red blood cells impacting their ability to respond to oxidative stress. This hospital-based cross-sectional study aimed at assessing the prevalence of G6PD deficiency in donor blood and the impact of the enzyme deficiency on red cell indices during storage.

Method

A total of 57 blood bags were screened for G6PD deficiency. Red cell indices and blood film comments were investigated on Day 0, Day 7 and Day 14 of storage.

Results

Eight out of 57 (14 %) had the G6PD full defect and 86 % (49/57) had no defect. Over the course of 14 days storage, the hemoglobin and red blood cell count significantly decreased in G6PD-deficient blood units with a corresponding significant increase in mean corpuscular volume and red cell distribution width-standard deviation compared to baseline and normal G6PD activity. The blood film comment showed 85.7 % normocytic normochromic, 2.0 % microcytic hypochromic and 12.2 % macrocytic hyperchromic from G6PD-non-deficient donors whereas G6PD-deficient donors had 75 % normocytic normochromic with 12.5 % microcytic hypochromic and 12.5 % macrocytic hypochromic after 2 wk in storage.

Conclusion

Red blood cell count and hemoglobin reduce significantly in G6PD-deficient donor units during storage with an associated increased mean corpuscular volume indicating progressive loss of the cellular membrane homeostatic mechanism that could potentially result in further hemolysis during long term storage.

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