糖醇乙酰化和液相色谱质谱法检测伯纳氏考克氏菌中独特的病毒糖和二氢羟基链糖

IF 2.4 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research Pub Date : 2025-05-10 DOI:10.1016/j.carres.2025.109524

Lawrence Webb , Stephen R. Graves , John Stenos , Karren M. Plain , Xavier A. Conlan

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引用次数: 0

摘要

伯纳氏克希菌引起人类q热和动物克希菌病。本工作提出了一种稳健、灵敏和选择性的方法来鉴定伯氏梭菌脂多糖中发现的两种独特的糖。糖通过糖醇乙酰化衍生，并通过高效液相色谱联用高分辨率质谱法进行鉴定。在衍生化过程中观察到病毒鼻糖和二氢羟基链糖仅部分乙酰化，这对于在缺乏商业标准的情况下鉴定这些糖是有价值的。这种灵敏的方法可以应用于诊断领域，并有助于进一步表征伯氏梭菌的细胞壁脂多糖。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Detection of the unique sugars virenose and dihydrohydroxystreptose from Coxiella burnetii using alditol acetylation and liquid chromatography mass spectroscopy

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Detection of the unique sugars virenose and dihydrohydroxystreptose from Coxiella burnetii using alditol acetylation and liquid chromatography mass spectroscopy

The bacterium Coxiella burnetii causes Q-Fever in humans and coxiellosis in animals. This work presents a robust, sensitive and selective method for the identification of two unique sugars found in the lipopolysaccharide of C. burnetii. The sugars were derivatised using alditol acetylation and identified using high performance liquid chromatography coupled to high resolution mass spectrometry. Virenose and dihydrohydroxystreptose were observed to be only partially acetylated during derivatisation and this was found to be valuable for the identification of these sugars in the absence of commercial standards. This sensitive methods can be applied in diagnostic fields and help to further characterise the cell-wall lipopolysaccharide of C. burnetii.

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来源期刊

Carbohydrate Research 化学-生化与分子生物学

CiteScore

5.00

自引率

3.20%

发文量

183

审稿时长

3.6 weeks

期刊介绍： Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".