硫酸盐过氧化物酶体氧化酶（SOX）是高等植物一氧化氮的替代来源，可被H2S上调

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-05-09 DOI:10.1016/j.plaphy.2025.110000

Francisco J. Corpas , Jorge Taboada , Beatriz Sánchez-Romera , Javier López-Jaramillo , José M. Palma

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引用次数: 0

摘要

一氧化氮（NO）是植物细胞内源性产生的一种自由基，尽管其酶促合成仍然是一个持续争论的主题。植物过氧化物酶体是一种亚细胞区室，具有活跃的硝基氧化代谢，在多种代谢途径中发挥作用。亚硫酸盐氧化酶（SOX）是一种需要钼辅助因子（MoCo）的过氧化物酶，它催化亚硫酸盐（SO32−）氧化为硫酸盐（SO42−），同时产生H2O2。利用从辣椒果实中提取的重组SOX酶，通过电子顺磁共振（EPR）和自旋捕获法检测，发现该酶具有以亚硝酸盐（NO2−）为底物、NADH为电子供体生成NO的能力。此外，•NO的生成在硫化氢（H2S）存在下上调，而在H2O2存在下下调，这突出了H2O2、•NO和H2S之间的关系。这一数据为理解高等植物中NO的酶促来源开辟了新的途径，特别是在过氧化物酶体中。

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Peroxisomal Sulfite Oxidase (SOX), an alternative source of NO in higher plants which is upregulated by H2S

Nitric oxide (^•NO) is a free radical that is endogenously produced in plant cells, though its enzymatic synthesis remains a subject of ongoing debate. Plant peroxisomes, subcellular compartments with active nitro-oxidative metabolism, play a role in various metabolic pathways. Sulfite oxidase (SOX), a peroxisomal enzyme requiring the molybdenum cofactor (MoCo), catalyzes the oxidation of sulfite (SO₃²⁻) to sulfate (SO₄²⁻), along with the concomitant production of H₂O₂. Using reconstituted recombinant SOX from pepper (Capsicum annuum L.) fruit, it was shown that this enzyme has the capacity to generate ^•NO using nitrite (NO₂⁻) as a substrate and NADH as an electron donor which was detected by electron paramagnetic resonance (EPR) spectroscopy coupled with the spin-trapping method. Furthermore, this ^•NO generation was upregulated in the presence of hydrogen sulfide (H₂S) but was downregulated by H₂O₂ which highlights the relationship between H₂O₂, ^•NO, and H₂S. This data opens new avenues for understanding the enzymatic sources of ^•NO in higher plants, particularly within peroxisomes.

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.