Upgraded multienzymes activities by Trichoderma reesei secretion using mixed Avicel and wheat bran as inducing carbon source

Plant cell wall–degrading enzymes produced by filamentous fungi play an important role in various biotechnological processes. The efficiency of lignocellulose hydrolysis was up to 1.9- to 2.7- fold when the mixture of Avicel and wheat bran was employed as the carbon source compared to that obtained with other soluble carbon sources. Furthermore, the protein complexes of the lignocellulase enzyme preparations were analyzed using polyacrylamide gel electrophoresis and mass spectrometry. The results showed that, when the mixture of Avicel and wheat bran was used as the carbon source, the protein complexes contained up to 7 different types of proteins, exhibiting significantly greater diversity in constituent proteins compared to those obtained using other carbon sources. Moreover, auxiliary proteins were detected as constituents of the multienzyme complexes in addition to hemicellulases and cellulases when the mixture of Avicel and wheat bran was employed as the carbon source. We hypothesize that these secreted multienzyme complexes consisting of cellulases, hemicellulases, and auxiliary proteins play an important role in enhancing lignocellulose degradation. The results help to deepen the understanding of fungal cellulases, thereby promoting the production technology progress and industrial application of filamentous fungal cellulase.