利用开源软件进行斑马鱼胚胎行为测试

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecules and Cells Pub Date : 2025-04-25 DOI:10.1016/j.mocell.2025.100221

Thilini Ranasinghe , Seon-Heui Cha

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引用次数: 0

摘要

这项工作描述了使用开源软件ImageJ1.54fFiji和VirtualDub2测量机车活动的简单方法。动物行为的意义是大脑活动的一面镜子，它可以提供与神经系统疾病有关的信息。商业行为分析软件经常需要专业知识，并且由于配备特定的工具来使用软件而花费高昂的成本，因此鼓励了向可访问且有效的开源替代方案发展的趋势。在这里，我们解释了如何转换视频格式，测量运动，并产生有用的机车参数，以帮助评估斑马鱼胚胎。这种方法可以很容易地转换为用于其他模型系统。这种方法旨在简化研究背景下的行为量化，鼓励更广泛的研究方面。

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Open-source software utilization for zebrafish embryos behavior test

This work described simple methods for measuring locomotive activity using open-source software, ImageJ1.54fFiji, and VirtualDub2. The significance of animal behavior is a mirror of brain activity, which can give information implicated with neurological diseases. Commercial behavioral analysis software frequently needs expertise and expenses high costs due to equip a specific instrument to use of software, thereby encouraging a trend toward open-source alternatives that are both accessible and effective. Here, we explained how to convert video format, measure movement, and produce useful locomotive parameters to aid in the assessment of zebrafish embryos. This method could be easily translated for use in other model systems. This methodology seeks to streamline behavioral quantification in research contexts, encouraging broader research aspects.

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来源期刊

Molecules and Cells 生物-生化与分子生物学

CiteScore

6.60

自引率

10.50%

发文量

审稿时长

2.3 months

期刊介绍： Molecules and Cells is an international on-line open-access journal devoted to the advancement and dissemination of fundamental knowledge in molecular and cellular biology. It was launched in 1990 and ISO abbreviation is "Mol. Cells". Reports on a broad range of topics of general interest to molecular and cell biologists are published. It is published on the last day of each month by the Korean Society for Molecular and Cellular Biology.